Optimizing conditions for treatment and extraction of collagen from fan-bellied leatherjacket skin Monacanthus chinensis (Osbeck, 1765)
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Author
Doan, Thi ThietCorporate Author
Pham, Xuan KyPhan, Bao Vy
Nguyen, Phuong Anh
Le, Ho Khanh Hy
Dao, Viet Ha
Vu, Ngoc Boi
Date
2020
Metadatos
Mostrar el registro completo del ítemAlternative Title
Nghiên cứu tối ưu hóa điều kiện xử lý và tách chiết collagen từ da cá bò gai móc Monacanthus chinensis (Osbeck, 1765)Abstract
The fan-bellied leatherjacket skin was treated to extract collagen by chemical method. The non-collagenous substances and the pigment in the skin were removed with NaOH and H2O2, respectively. Collagen was extracted with acetic acid. The treatment and extraction conditions were optimized by response surface methodology. The concentrations, skin/solution ratios (g/ml) and times were surveyed. The optimum conditions to remove the non-collagenous substances were as follows: the concentration of NaOH at 0.15M, the ratio (g/ml) at 1/13.9, time at 56.9 hours. Using H2O2 with the concentration at6%, the skin/solution ratio (g/ml) at 1/2, time at 10 minutes were suitable values for skin pigmentation removal. For collagen extraction, the concentration of acetic acid at 0.53M, skin/solution ratio at 1/9.6 g/ml and time in 59.6 hours were optimal.Journal
Collection of Marine Research WorksVolume
25Issue/Article Nr
Special issue to introduce scientific papers from the Collection of Marine Research Works, Volume 25 published in Vietnam Journal of Marine Science and Technology No. 4A(T20), 2020Page Range
pp. 141-154Resource/Dataset Location
https://doi.org/10.15625/1859-3097/15657