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dc.contributor.authorPourkazemi, M.
dc.contributor.authorRazikazemi, S.
dc.coverage.spatialIranen_US
dc.coverage.spatialCaspian Seaen_US
dc.date.accessioned2017-11-17T07:25:13Z
dc.date.available2017-11-17T07:25:13Z
dc.date.issued2011
dc.identifier.issn1735-3033
dc.identifier.urihttp://hdl.handle.net/1834/10411
dc.description.abstractIn order to identify the sex marker in Mahisefied, Rutilus frisii kutum, samples from 5 male and 5 female fish were collected from the south Caspian Sea. Polymerase chain reaction random amplified polymorphic DNA (PCR-RAPD) was performed using 124 primer sets. All bands were numbered using 1 and 0 scores corresponding to the presence or absence of bands, respectively and data were analyzed using RAPDPLOT program. Results indicated that 44 sets of primers did not show any flanking site and produced no bands, while the remaining 80 produced sharp and visible bands on polyacrylamid gel. In total, 1600 bands were scored. However, none of the bands corresponded to either the male or female fish. According to the results it has been concluded that RAPD technique failed to detect sex and cannot be considered as a robust molecular tool for sex differentiation in the studied fish. The reason may be the absence of sex chromosomes in this species or that the genes corresponding to sex differentiation are spread on different autosomal chromosomes with interaction of some environmental factors.en_US
dc.language.isoenen_US
dc.subject.otherRutilus frisii kutumen_US
dc.subject.otherMahisefiden_US
dc.subject.otherRandom Amplified Polymorphic DNAen_US
dc.subject.otherSex determinationen_US
dc.titleFailure of PCR-RAPD technique to differentiate sex in Mahisefied (Rutilus frisii kutum) from the South Caspian Seaen_US
dc.typeJournal Contributionen_US
dc.bibliographicCitation.issue2en_US
dc.bibliographicCitation.titleCaspian Journal of Environmental Sciencesen_US
dc.bibliographicCitation.volume9en_US
dc.description.statusPublisheden_US
dc.format.pagerangepp.235-242en_US
dc.subject.asfaFailureen_US
dc.subject.asfaSexen_US
dc.subject.asfaDNAen_US
dc.type.refereedRefereeden_US
refterms.dateFOA2021-01-30T18:48:38Z


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