Effect of LHRHA2 and carp pituitary extract hormone on some spermatozoa fertility and quality indices in Barbus sharpeyi

Abstract

Effects of LHRHA2 hormone in 2.5, 5 and 10µg/kg doses and anti-dopamine metoclopramide on Barbus sharpeyi spermiation, spermatozoa quality and fertility indices, chemical (Na+ , K+ and Ca+2) and biochemical component (Alkaline phosphates, Glucose and Triglyceride) of seminal plasma and its osmotic pressure were assessed in comparison to Carp pituitary extract treatment. Results indicated that the highest incubation survival, hatching rate, sperm volume, duration of sperm motility as well as the lowest larval deformity were achieved by injection of LHRHA2 (10µg/kg +2.5mg/kg metoclopramide) and it was significantly different to others groups. Application of above mentioned treatment had the highest osmotic pressure with about (Mean ±SD) 324±7.31mOsmol kg-1 among other groups. Also, the highest level of Na+ was assessed by injection of LHRHA2 (10µg/kg +2.5mg/kg metoclopramide). Result showed that injection of Carp pituitary extract caused the highest spermatocrit, K+ value and larval deformity in comparison to other treatments. In conclusion, optimum sperm quality can be produced by injection of LHRHA2 (10µg/kg + 2.5mg/kg metoclopramide) in males of B. sharpeyi at 8 hour following hormonal stimulation. Also, above mentioned treatment caused increasing of incubation survival and decrease of larval deformity in B. sharpeyi.