The effects of glucan on hematological parameters, immune response and intestinal microbiota of Rutilus frisii kutum fry

Abstract

The purpose of this study was to determine the effect of dietary glucan on some haematological parameters, immune response and intestinal microbiota of Rutilus frisii kutum. In the present study, various levels of ingredient so called Hoplit (0, 0.5, 1.0, 1.5, and 2.0%) containing glucan was added to a basal formulated diet. Twenty and five kutum fry with a mean (±SD) weight of 1.15±0.06g were stocked in each experimental tank (100 l capacity) filled with 80 liter of water. Fish were fed on experimental diet for 56 days and biometry was performed every 15 days. At the end of the trial blood samples were collected for measurement of haematological parameters including: Red and white blood cells count, differential count of white blood cells, hematocrit and hemoglobin, and innate immune factors (Immunoglobulin and Lysozyme). Fries in 1.5% treatment had highest serum immunoglobins (Ig) and eosinophil, although when compared with control but with no significant differences. Highest MCHC, hematocrit and hemoglobin were observed in the 0.5 and 1% treatments and the highest MCV and red blood cell count were in 0.5 percent treatment. The highest white blood cells count and neutrophils was observed in 2% treatment. The highest and lowest levels of lysozyme activity were observed in 1% and control treatments, respectively. Evaluation of the total bacteria and LAB counts revealed significant increase in 0.5% treatment. According to these results administration of dietary glucan can be considered for stimulation of innate immune response of white fish fry.