The study of Total Hemocyte Count and Total Protein Plasma in Shrimp Litopenaeus vannamei infected with Monodon baculovirus

Abstract

The investigation of Total Hemocyte Count (THC) and Total Protein Plasma (TPP) in shrimp juvenile Litopenaeus vannamei that exposed with Monodon Baculovirus (MBV) was carried out. Three hundred and sixty shrimps with average weight 7 to 12 g were selected from Heleh site in Bousher province and transported to Iran Shrimp Research Institute. The shrimp was acclimated during two days for stress reduction and adaptation. The shrimps were cheeked by PCR for WSSV, MBV, HPV, TSV, IHHNV and NHP and the result showed they are free of these diseases. The experiment was designed with selected 60 shrimps in triplicate as treatment and exposed with MBV during 12 hours and control group with triplicate without exposing with MBV. The hemolymph was withdrawn from abdominal segments of samples for measuring THC and TPC evaluation at designed hours (0, 12, 24, 48, 72 and 96). The PCR test confirmed the MBV in treatment and the control were free of viruses. The results showed that the difference of THC and TPP value between treatment and control group during the experiment was significant (P < 0.05). This finding can be used for assessing the health of shrimp culture and prevention of MBV.