The control of recovery process with clove tree (Eugenia caryophyllata) in rainbow trout (Oncorhynchus mykiss)

Abstract

In order to investigate the effect of different temperature of anesthetic (clove tree) and recover (anesthetic-free water) solutions on recovery process, The anesthetic induction times and recovery times of 540 fingerlings of rainbow trout (Oncorhynchus mykiss) weighing 3.2 ± 0.2 g in anesthesia/ recovery solutions with cross-combined of temperature of 5,11 and 17 °C as experimental treatments (9 treatments) were compared. The anesthesia was induced by170 ppm of aqueous clove (Eugenia caryophyllata) solution. Results showed that both induction and recovery times were adversely temperature-dependent. Recovery times of treatments, related to temperature of anesthetic solutions and their temperature, were significantly different (P < 0.05). Our results indicated that mean recovery time (three temperature treatments) of the fish exposed to the lower temperature anesthetic solutions generally decreased opposing with higher anesthetic solutions. The lowest recovery time (61±11.1 seconds) in17°C anesthetic-free water occurred in fishes induced to 5°C anesthetic solution. In contrast, the highest recovery time (523±154 seconds) in 5°C water happened in fishes induced to 17°C anesthetic solution. Warm and cold thermal difference in anesthetic and recover solutions could extend recovery time 1 up to 8 minutes. Regarding to aim and demanded time, by selected favorite temperatures can control anesthesia/or recovery time in order to prevent the use of drug over-dosing.