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Extraction and Identification Sterols in Brown alga, Padina boergesenii in Chabahar Coasts

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Author
Jamili, Sh.
Gohari Kakhki, A.R.
Saeidnia, S.
Permeh, P.
Date
2015

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Abstract
Padina boergesenii is one of the most abundant brown algae distributed in the north of Persian Gulf and Oman Sea. In this study after sampling and preparation of Padina boergesenii by Chroform-Etanol (3-1) solvent and by Methanol has been extract. Separation and purification of the compounds was carried out using thin layer, general and inverse column chromatography, Cephadex and high-performance liquid chromatography (HPLC). Structural elucidation of the constituents was based on the data obtained from H-NMR, 13C-NMR, HSQC, HMBC, DEPT and Cephadex LH-20. The steroids compounds separated from above alga were identified as 22-dehydrocholesterol (1), cholesterol (2), fucosterol (3), β-sitosterol (4), stigmasterol (5), ostreasterol (6) and two epimer of hyroxyestrol(7), based on their spectral data and from comparison with those previously reported in the literature.
Journal
Iranian Scientific Fisheries Journal
Volume
24
Issue/Article Nr
3
Page Range
pp.35-44
URI
http://hdl.handle.net/1834/10858
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Iranian Scientific Fisheries Journal

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