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dc.contributor.authorKhafaeizadeh, K.
dc.contributor.authorSakhaei, N.
dc.contributor.authorDoustshenas, B.
dc.contributor.authorGhanemi, K.
dc.contributor.authorZolgharnein, H.
dc.coverage.spatialIranen_US
dc.date.accessioned2017-12-22T12:28:18Z
dc.date.available2017-12-22T12:28:18Z
dc.date.issued2016
dc.identifier.issn1026-1354
dc.identifier.urihttp://hdl.handle.net/1834/10876
dc.description.abstractRotifers are an important group of zooplankton in aquatic ecosystems that contain relatively high amounts of unsaturated fatty acids, proteins and peptides. Rotifers, especially Brachionus plicatilis species, are one of the important live food sources for maine fish larvae in aquaculture. The aim of this study was to evaluate the antioxidant activities of purified peptides from B. plicatilis. Antioxidant peptids of the B. plicatilis have been hydroylzed by Alcalase, α­Chymotrypsin, Papain, Neutrase, Pepsin, and Trypsin. Their antioxidant activity were evaluated by the free radical inhibitpory effect of diphenylpicrylhydrazyl (DPPH). Also the sequential chromatography method was used for extraction and purification of the peptides. The results showed that peptides obtained from the pepsin hydrolysate have a higher inhibitory effect than other peptides. Pepsin showed 58% inhibitory effect in 0.015 mM DPPH. Finally, the pepsin hydrolyzate constitutes were purified and isolated by gel-filtration chromatography (Sephadex G-250) and reverse-phase liquid chromatography on Eurospher C18 column (250×4.6 mm), respectively. The results of this study have been recognized the high antioxidant activities of extracted hydrolysates from B. plicatilis and their feasibility of using them in food industries as a food complement.en_US
dc.language.isofaen_US
dc.subject.otherRotifersen_US
dc.subject.otherDPPHen_US
dc.subject.otherAntioxidant peptidesen_US
dc.subject.otherHydrolysisen_US
dc.subject.otherPepsinen_US
dc.subject.otherBrachionus plicatilisen_US
dc.subject.otherB. plicatilisen_US
dc.titleEvaluation of antioxidant activity of the purified peptides from hydrolysis of rotifer (Brachionus plicatilis)en_US
dc.typeJournal Contributionen_US
dc.bibliographicCitation.issue2en_US
dc.bibliographicCitation.titleIranian Scientific Fisheries Journalen_US
dc.bibliographicCitation.volume25en_US
dc.description.statusPublisheden_US
dc.format.pagerangepp.69-78en_US
dc.subject.asfaEnzymesen_US
dc.subject.asfaEvaluationen_US
dc.subject.asfaZooplanktonen_US
dc.subject.asfaFooden_US
dc.subject.asfaProteinen_US
dc.type.refereedRefereeden_US
refterms.dateFOA2021-01-30T18:48:27Z


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