17α-Hydroxy-4-pregenen-3-one (17 αP) assay, using acetylcholinesterase enzyme as tracer
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Author
Ebrahimi, M.Date
2005
Metadata
Show full item recordAbstract
17a-hydroxy-4-pregnen-3-one (17aP) hormone is a precursor of other steroid hormones and radioimmunoassay has already been used to measure it, but a simple and rapid "Enzyme Linked Immunosorbant Assay" (ELISA) is described and validated here. A general procedure for prepara-tion of the acetylcholinesterase labelled steroid is described, which is applicable to any steroid. Use of acetylcholinesterase tracer increased the sensitivity of assay so that reliable measurements of each steroid could be achieved with only 10 ml of plasma. The ELISA was applied to measure 17, 20a-hydroxy-4-pregnen-3-one (17, 20aP) steroid production from 17aP by 20 a-hydroxy steroid dehydrogenase (20a-HSD) from sperm of roach (Rutilus rutilus). The results showed that cyprinid sperm contains potent and active 20a-HSD enzymes which produce 17, 20aP hormone from 17aP substrate.Journal
Iranian Journal of Fisheries ScienceVolume
5Issue/Article Nr
1Page Range
pp.13-28Collections