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dc.contributor.authorEbrahimi, M.
dc.coverage.spatialIranen_US
dc.coverage.spatialQomen_US
dc.date.accessioned2018-02-09T11:43:30Z
dc.date.available2018-02-09T11:43:30Z
dc.date.issued2005
dc.identifier.issn1562-2916
dc.identifier.urihttp://hdl.handle.net/1834/11130
dc.description.abstract17a-hydroxy-4-pregnen-3-one (17aP) hormone is a precursor of other steroid hormones and radioimmunoassay has already been used to measure it, but a simple and rapid "Enzyme Linked Immunosorbant Assay" (ELISA) is described and validated here. A general procedure for prepara-tion of the acetylcholinesterase labelled steroid is described, which is applicable to any steroid. Use of acetylcholinesterase tracer increased the sensitivity of assay so that reliable measurements of each steroid could be achieved with only 10 ml of plasma. The ELISA was applied to measure 17, 20a-hydroxy-4-pregnen-3-one (17, 20aP) steroid production from 17aP by 20 a-hydroxy steroid dehydrogenase (20a-HSD) from sperm of roach (Rutilus rutilus). The results showed that cyprinid sperm contains potent and active 20a-HSD enzymes which produce 17, 20aP hormone from 17aP substrate.en_US
dc.language.isoenen_US
dc.subject.otherRutilus rutilusen_US
dc.title17α-Hydroxy-4-pregenen-3-one (17 αP) assay, using acetylcholinesterase enzyme as traceren_US
dc.typeJournal Contributionen_US
dc.bibliographicCitation.issue1en_US
dc.bibliographicCitation.titleIranian Journal of Fisheries Scienceen_US
dc.bibliographicCitation.volume5en_US
dc.description.statusPublisheden_US
dc.format.pagerangepp.13-28en_US
dc.subject.asfaELISAen_US
dc.subject.asfaSteoidsen_US
dc.subject.asfaTEROIDSen_US
dc.subject.asfaAcetylcholinesteraseen_US
dc.subject.asfaRoachen_US
dc.subject.asfa17α-hydroxy-4-pregnen-3-one assayen_US
dc.type.refereedRefereeden_US
refterms.dateFOA2021-01-30T18:48:17Z


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