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dc.contributor.authorPourkazemi, M.
dc.contributor.authorAsadyan, H.
dc.contributor.authorPazooki, J.
dc.contributor.authorMasoumian, M.
dc.contributor.authorEbrahimzadeh Mossavi, H.A.
dc.coverage.spatialIranen_US
dc.coverage.spatialKhouzestan Provinceen_US
dc.coverage.spatialGilan Provinceen_US
dc.date.accessioned2018-02-15T08:34:13Z
dc.date.available2018-02-15T08:34:13Z
dc.date.issued2007
dc.identifier.issn1026-1354
dc.identifier.urihttp://hdl.handle.net/1834/11311
dc.description.abstractMolecular comparison of two parasites Lernaea cyprinacea and Lernaea ctenopharyngodoni was carried out using RAPD (Random Amplified Polymorphic DNA) technique. A total of 43 Lernaea specimens belonging to the two species were collected from the Guilan and Khouzestan Provinces. DNA was extracted using the Phenol-chloroform method. The quality and quantity of DNA was assessed using 1% Agarose gel electrophoresis and spectrophotometer. Polymerase Chain Reaction (PCR) was conducted on the target DNA under specific conditions and PCR products were subjected to electrophoresis on polyacrylamide gels (6%). Polyacrylamide gels were stained using silver nitrate and DNA bands were analyzed with BioCapt software. The genetic analysis was conducted using POP GEN 32 software. Forty two primers, 10 nucleotides each were used for PCR reaction. Totally, 397 RAPD loci were counted on polyacrylamide gel where 349 identical loci were polymorphic of which some bands may be used as genetic markers for the identification of both Lernaea species. Data analysis on PCR products showed higher genetic variation (1.15%) of L. Ctenopharyngodon in the Guilan Province as compared to that of the Khouzestan (0.0%). However, genetic variation (27.46%) of L. cyprinacea in the Khouzestan province was 7.26 times higher than that of the Guilan province (3.78%). The two species showed a genetic differentiation of approximately 88%. Based on the observed molecular differences, we state that L. ctenopharyngodoni is a genetically independent species from L. cyprinacea.en_US
dc.language.isofaen_US
dc.relation.urihttp://isfj.areo.ir/en_US
dc.subject.otherParasitesen_US
dc.subject.otherPolymerase chain reactionen_US
dc.subject.otherPrimersen_US
dc.subject.otherNucleotidesen_US
dc.subject.otherLernaea ctenopharyngodonien_US
dc.subject.otherLernaea cyprinaceaen_US
dc.titleIntroducing molecular markers for Lernaea cyprinacea and Lernaea ctenopharyngodoni using RAPD techniqueen_US
dc.typeJournal Contributionen_US
dc.bibliographicCitation.issue3en_US
dc.bibliographicCitation.titleIranian Scientific Fisheries Journalen_US
dc.bibliographicCitation.volume16en_US
dc.description.statusPublisheden_US
dc.format.pagerangepp.19-28en_US
dc.subject.asfaMolecular structureen_US
dc.subject.asfaGeneticsen_US
dc.subject.asfaDNAen_US
dc.subject.asfaFreshwateren_US
dc.type.refereedRefereeden_US
refterms.dateFOA2021-01-30T18:48:22Z


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