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dc.contributor.authorKakoolaki, S.
dc.contributor.authorSoltani, M.
dc.contributor.authorEbrahimzadeh Mousavi, H. A.
dc.contributor.authorSharifpour, I.
dc.contributor.authorMirzargar, S.
dc.contributor.authorAfsharnasab, M.
dc.contributor.authorMotalebi, A. A.
dc.coverage.spatialIranen_US
dc.coverage.spatialTehranen_US
dc.date.accessioned2018-02-16T10:00:04Z
dc.date.available2018-02-16T10:00:04Z
dc.date.issued2011
dc.identifier.issn1562-2916
dc.identifier.urihttp://hdl.handle.net/1834/11387
dc.description.abstractShrimp farmers have been suffering from loss of production due to White Spot Disease (WSD) in the last decades. A few studies focused on the environmental factors predisposed WSD. Our study aimed to investigate the effects of the different salinities on occurrence of WSD. The treatments were 30 ppt, 40 ppt and 50 ppt in triplicate and corresponding untreated control groups. White Spot Virus (WSV) was intramuscularly injected to the shrimp in whole treatments. PCR and histopathological findings within the measurement of some hemolymph parameters such as Differentiated Hemocyte Count (DHC), Total Hemocyte Count (THC) and Total Plasma Protein (TPP) were carried out. A positive result was observed for the occurrence of the antigen of the WSV obtained from the samples of each treatment of salinity due to the challenging treatments with the WSV. Mortality at the salinity of 30 ppt began at 72 h post inoculation as well as the salinity of 40 ppt, but at 50 ppt it started after 50 h. The Minimum and maximum count of mortality at the salinity of 30, 40 and 50 ppt were 3.5, 8.5 0.5, 4.5 and 1.5, 7.5, respectively. No mortality was observed in the untreated control groups of 30, 40 and 50 ppt during the experiment. It is concluded that the higher and the lower salinity, lesser or greater than the normal condition in exposed to WSV could lead to severe mortality of WSD.en_US
dc.language.isoenen_US
dc.titleThe effect of different salinities on mortality and histopathological changes of SPF imported Litopenaeus vannamei, experimentally exposed to White Spot Virus and a new differential hemocyte staining methoden_US
dc.typeJournal Contributionen_US
dc.bibliographicCitation.issue3en_US
dc.bibliographicCitation.titleIranian Journal of Fisheries Scienceen_US
dc.bibliographicCitation.volume10en_US
dc.description.statusPublisheden_US
dc.format.pagerangepp.447-460en_US
dc.subject.asfaWSDen_US
dc.subject.asfaSalinityen_US
dc.subject.asfaLitopenaeus vannameien_US
dc.subject.asfaTHCen_US
dc.subject.asfaTPPen_US
dc.subject.asfaDHCen_US
dc.subject.asfaMortalityen_US
dc.type.refereedRefereeden_US
refterms.dateFOA2021-01-30T18:48:17Z


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