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dc.contributor.authorDadar, M.
dc.contributor.authorRajabi Memari, H.
dc.contributor.authorPeyghan, R.
dc.contributor.authorSeifi Abad Shapouri, M.R.
dc.coverage.spatialIranen_US
dc.date.accessioned2018-02-27T14:09:34Z
dc.date.available2018-02-27T14:09:34Z
dc.date.issued2014
dc.identifier.issn1562-2916
dc.identifier.urihttp://hdl.handle.net/1834/11795
dc.description.abstractInfectious Pancreatic Necrosis Virus (IPNV) is a member of the family Birnaviridae that has been linked to high mortalities in salmonids. Bacterial based systems as live vectors for the delivery of heterologous antigens offer a number of advantages as vaccination strategies. VP2 is a structural viral protein of IPNV with immunogenicity effects. In this study IPNV was isolated from diseased fry of rainbow trout Oncorhynchus mykiss (Walbaum) using CHSE-214. Then an expression vector was constructed for expression of viral protein VP2. The designed vector was constructed based upon pET-26b (+) with T7 promoter. A fragment containing the full length of the VP2 gene of Iranian Sp strain was amplified by PCR using genomic RNA of IPNV as template and cloned inpET-26b(+) plasmid. Recombinant structural viral protein VP2 was expressed as a soluble, N-terminal PelB fusion protein and secreted into the periplasmic space of Escherichia coli BL21(DE3) and Rosetta (DE3). The glucose, Isopropyl-β-D- thiogalactopyranoside (IPTG) was used as a chemical inducer for rVP2 production in 37º C. The rVP2 was extracted from the periplasm by osmotic shock treatment. The presence of gene in bacterial system of E. coli was confirmed by gel electrophoresis technique. The constructed vector could efficiently express the rVP2 into the periplasmic space of E. coli. The successful cloning and expression of the structural viral protein gene into E. coli can be used for developing a useful and safe vaccine to control IPNV infection in Iranian fish industry.en_US
dc.language.isoenen_US
dc.subject.otherFish diseaseen_US
dc.subject.otherOncorhynchus mykissen_US
dc.titleIsolation and expression of recombinant viral protein (VP2) from Iranian isolates of Infectious Pancreatic Necrosis Virus (IPNV) in Escherichia colien_US
dc.typeJournal Contributionen_US
dc.bibliographicCitation.issue4en_US
dc.bibliographicCitation.titleIranian Journal of Fisheries Scienceen_US
dc.bibliographicCitation.volume13en_US
dc.description.statusPublisheden_US
dc.format.pagerangepp.856-868en_US
dc.subject.asfaVP2en_US
dc.subject.asfaInfectious Pancreatic Necrosis Virusen_US
dc.subject.asfaRecombinant viral proteinen_US
dc.subject.asfaPeriplasmic spaceen_US
dc.subject.asfaEscherichia colien_US
dc.type.refereedRefereeden_US
refterms.dateFOA2021-01-30T18:48:16Z


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