Show simple item record

dc.contributor.authorMalekinejad, H.
dc.contributor.authorAgh, N.
dc.coverage.spatialIranen_US
dc.date.accessioned2018-03-04T12:50:20Z
dc.date.available2018-03-04T12:50:20Z
dc.date.issued2016
dc.identifier.issn1562-2916
dc.identifier.urihttp://hdl.handle.net/1834/11986
dc.description.abstractZearalenone (ZEA) as mycoestrogen is found in human foods and animal feeds. Its estrogenic potency depends on its biotransformation fate. The hepatic biotransformation of ZEA in two species of sturgeon fish (Acipenser persicus and Huso huso) was investigated. ZEA was incubated with the hepatic microsomal and post-mitochondrial sub-fractions in the presence of NADPH and the metabolites were determined by means of HPLC. Moreover, the rate of glucuronidation for ZEA and its metabolites were estimated in the presence of uridine diphosphateglucuronic acid. b-zearalenol (b-ZOL) was found to be the major metabolite of ZEA by both sub-fractions. Enzymatic kinetics studies revealed that the maximum velocity (Vmax) in microsomal and post-mitochondrial fractions for b-ZOL production was found 5- and 7-folds in Huso huso and 8- and 12-folds in A. persicus higher than that for a-ZOL production, respectively. The H. huso hepatic post-mitochondrial fraction mainly glucurinated ZEA while in A. persicus, the metabolites and in particular b-ZOL were glucuronidated. Data suggest that the hepatic biotransformation of ZEA in studied sturgeons resulted in detoxification of ZEA as the main metabolite tends to be b-ZOL with weaker estrogenic property. Moreover, clear differences in glucuronidation profile are indicating interspecies variety in hepatic biotransformation of ZEA.en_US
dc.language.isoenen_US
dc.subject.otherFish diseaseen_US
dc.titleInterspecies variation in the hepatic biotransformation of zearalenone: Evidence for bio-inactivation of mycoestrogen zearalenone in sturgeon fishen_US
dc.typeJournal Contributionen_US
dc.bibliographicCitation.issue1en_US
dc.bibliographicCitation.titleIranian Journal of Fisheries Scienceen_US
dc.bibliographicCitation.volume15en_US
dc.description.statusPublisheden_US
dc.format.pagerangepp.415-425en_US
dc.subject.asfaAcipenser persicusen_US
dc.subject.asfaHuso husoen_US
dc.subject.asfaHepatic biotransformationen_US
dc.subject.asfaGlucuronidationen_US
dc.subject.asfaSubcellular fractionsen_US
dc.subject.asfaZearalenoneen_US
dc.subject.asfaFishen_US
dc.type.refereedRefereeden_US
refterms.dateFOA2021-01-30T18:48:22Z


Files in this item

Thumbnail
Name:
IFRO-v15n1p415-en.pdf
Size:
436.6Kb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record