The effects of Glycerinate Hypophysis injection on the fluctuation of steroid hormones on Persian sturgeon (Acipenser persicus)

Abstract

Using glycerin as a solvent of hypophysis powder for inducing ovulation in Acipencer persicus was studied at Shahid Beheshti Sturgeon Fish Reproduction Complex and Internatinal Sturgeon Research Institue. The experiment was done using four treatments in five replications. In treatment one, the female fish were injected with glycerin, whereas in treatment two, physiologic serum was used for injection. The females in treatment three were given two injections of glycerin, while those of the treatment four received two injections of physiologic serum. The evaluated factors in the blood serum included 17- 13 estradiol and 17-a hydroxy progesterone. The biometry factors were measured as well. The fish in treatment one showed higher frequency of fertilization, more concentration of estradiol and progesterone hormones compared to other treatments, although insignificantly. Treatment with one injection of physiologic serum caused the lowest amount of these factors. The fluidity of ovules in the glycerin treatments were more than physiologic-serum treatments. The ovulation time in the first treatment was shortest. A statistically significant sudden increase in progesterone was detected after hypophysis injection (P<0.000). A high correlation between the temperature and the maturation time was observed (P=0.0001). The temperature showed a significant correlation with increase in progesterone level (P=0.005). The results indicated that glycerinated hypophsis is a more suitable substance for inducing ovulation than physiologic serum.