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dc.contributor.authorGao, M.L.
dc.contributor.authorHou, H.M.
dc.contributor.authorZhang, G.L.
dc.contributor.authorLiu, Y.
dc.contributor.authorSun, L.M.
dc.coverage.spatialChinaen_US
dc.date.accessioned2018-03-06T12:34:57Z
dc.date.available2018-03-06T12:34:57Z
dc.date.issued2017
dc.identifier.issn1562-2916
dc.identifier.urihttp://hdl.handle.net/1834/12161
dc.description.abstractThe intestinal bacterial diversity of Stichopus japonicus was investigated using 16S ribosomal RNA gene (rDNA) clone library and Polymerase Chain Reaction/Denaturing Gradient Gel Electrophoresis (PCR-DGGE). The clone library yielded a total of 188 clones, and these were sequenced and classified into 106 operational taxonomic units (OTUs) with sequence similarity ranging from 88 to 100%. The coverage of the library was 77.4%, with approximately 88.7% of the sequences affiliated to Proteobacteria. Gammaproteobacteria and Vibrio sp. were the predominant groups in the intestine of S. japonicus. Some bacteria such as Legionella sp., Brachybacterium sp., Streptomyces sp., Propionigenium sp. and Psychrobacter sp were first identified in the intestine of sea cucumber.en_US
dc.language.isoenen_US
dc.subject.otherBiologyen_US
dc.subject.otherPhysiologyen_US
dc.subject.otherProteobacteriaen_US
dc.subject.otherGammaproteobacteriaen_US
dc.subject.otherVibrio sp.en_US
dc.titleBacterial diversity in the intestine of sea cucumber Stichopus japonicusen_US
dc.typeJournal Contributionen_US
dc.bibliographicCitation.issue1en_US
dc.bibliographicCitation.titleIranian Journal of Fisheries Scienceen_US
dc.bibliographicCitation.volume16en_US
dc.description.statusPublisheden_US
dc.format.pagerangepp.318-325en_US
dc.subject.asfaIntestinal bacterial diversityen_US
dc.subject.asfa16S rDNAen_US
dc.subject.asfaPCR-DGGEen_US
dc.subject.asfaSequencingen_US
dc.subject.asfaStichopus japonicusen_US
dc.type.refereedRefereeden_US
refterms.dateFOA2021-01-30T18:48:22Z


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