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dc.contributor.authorNajjar Lashgari, S.
dc.contributor.authorRezvni Gilkolaei, S.
dc.contributor.authorKamali, A.
dc.contributor.authorSoltani, M.
dc.coverage.spatialIranen_US
dc.coverage.spatialSardabrud riveren_US
dc.coverage.spatialAstara riveren_US
dc.date.accessioned2018-03-07T12:29:24Z
dc.date.available2018-03-07T12:29:24Z
dc.date.issued2017
dc.identifier.issn1562-2916
dc.identifier.urihttp://hdl.handle.net/1834/12166
dc.description.abstractIn this study the genetic diversity of wild Caspian trout (Salmo trutta caspius) in the Sardabroud and Astara Rivers was evaluated using D- Loop region sequencing. For this purpose, 35 specimens of adult Caspian brown trout were collected from these rivers in the Mazandarn and Gilan Provinces in fall and winter 2011. Approximately 3-5 g of soft and fresh fin tissue was isolated and fixed in ethanol 96% and then transferred to the Caspian Sea Ecology Research Center Genetics Laboratory in Sari, Iran. Genomic DNA from the samples was extracted using Ammonium Acetate Method. The quality and quantity of the extracted DNA were assessed by spectrophotometer and agarose gel (1%) electrophoresis. Polymerase Chain Reaction (PCR) was performed on the target DNA using a primers sequence D- Loop region of mtDNA molecule. Then the product was purified and DNA sequencing was carried out using chain termination method. The D- Loop region of Caspian trout contained 654 bp. Data were analyzed using Bio-Edit, DnaSP, Arlequin and Mega software. 20 and 15 haplotypes was observed in Sardabrud and Astara River. Tthe DNA sequence of one of them was recorded in Gene Bank with numbers KC991027 and KF015727. 223 and 240 polymorphic loci were detected in Sardabrud and Astara River that all of them were out of Hardy- Weinberg equilibrium (p<0.05). Average nucleotide and haplotype diversity were 0.127±0.067, 1.000±0.005 in Sardabrud River and 0.118±0.063 and 1.000±0.005 in Astara River.en_US
dc.language.isoenen_US
dc.subject.otherAquacultureen_US
dc.titleStudy of genetic diversity of wild Caspian trout Salmo trutta caspius in the Sardabrud and Astara Rivers, using D- Loop region sequencingen_US
dc.typeJournal Contributionen_US
dc.bibliographicCitation.issue1en_US
dc.bibliographicCitation.titleIranian Journal of Fisheries Scienceen_US
dc.bibliographicCitation.volume16en_US
dc.description.statusPublisheden_US
dc.format.pagerangepp.352-365en_US
dc.subject.asfaGenetic diversityen_US
dc.subject.asfaSalmo trutta caspiusen_US
dc.subject.asfaSequencingen_US
dc.type.refereedRefereeden_US
refterms.dateFOA2021-01-30T18:48:17Z


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