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dc.contributor.authorKarimzadeh, K.
dc.contributor.authorMostafaie, A.
dc.contributor.authorEsmaeli, A.
dc.contributor.authorPoorkazemi, M.
dc.contributor.authorZahmatkesh, A.
dc.coverage.spatialIranen_US
dc.date.accessioned2018-03-08T13:30:50Z
dc.date.available2018-03-08T13:30:50Z
dc.date.issued2005
dc.identifier.issn1026-1354
dc.identifier.urihttp://hdl.handle.net/1834/12220
dc.description.abstractCytochrome P4501 AI is a major isoenzyme in fish monooxygenase system which is induced by polycyclic aromatic hydrocarbons (PAHs) compounds. In this research, the inducing effect of p-naphtoflavone and its catalytic properties was studied in Huso huso liver. Fish were given ip injection of p-naphthoflavone at three different doses. The enzyme activity was measured with de-ethylation of ethoxyresorufin reaction (EROD) by flourometery method and relative amount of induced proteins were determined using polyacrylamid del electrophoresis (SDS-PAGE). The results showed that EROD activity in the microsomal fraction of the treated fish was 15-26 folds that of the control group. Optimum activity of this enzyme was observed at 20-25 degrees centivade. The maximum enzyme activity was seen in the precence of 180 micrograms of microsomai protein and 1.5311M of 7-ethoxyresorufin. SDS-PAGE of microsomal protein pattern in the treated fish revealed a protein with molecular mass 58+1 KDa translating to cytochrome P4501A. We conclude that the p-naphtoflovone in fish liver can induce cytochrome P4501A gene and increase its biosenthysis leading to raised enzyme activity in EROD reaction.en_US
dc.language.isofaen_US
dc.relation.urihttp://isfj.areo.ir/en_US
dc.subject.otherHuso husoen_US
dc.titleInduction of Cytochrome P4501A1 by beta-naphtho flavone and determination of enzyme properties in Huso husoen_US
dc.typeJournal Contributionen_US
dc.bibliographicCitation.issue2en_US
dc.bibliographicCitation.titleIranian Scientific Fisheries Journalen_US
dc.bibliographicCitation.volume14en_US
dc.description.statusPublisheden_US
dc.format.pagerangepp.115-126en_US
dc.subject.asfaCytochrome P4501A Ien_US
dc.subject.asfaBeta-naphtho flavoneen_US
dc.type.refereedRefereeden_US
refterms.dateFOA2021-01-30T18:48:22Z


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