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dc.contributor.authorNahavandi, R.
dc.contributor.authorRezvani, S.
dc.contributor.authorVosoughi, Gh.
dc.contributor.authorKazemi, B.
dc.coverage.spatialIranen_US
dc.coverage.spatialPersian Gulfen_US
dc.coverage.spatialOman Seaen_US
dc.date.accessioned2018-03-08T13:38:43Z
dc.date.available2018-03-08T13:38:43Z
dc.date.issued2005
dc.identifier.issn1026-1354
dc.identifier.urihttp://hdl.handle.net/1834/12257
dc.description.abstractWe used PCR-RFLP method to identify cuttlefish (Sepia pharaonis) populations in the Persian Gulf and the Sea of Oman. Bottom trawling method was used to collect a range of 20 to 40 specimens from each 15 stations in the study area. Genomic DNA was extracted by phenol-chloroform method and one pair primer was designed for the analysis based on 1 Ss rRNA gene nucleotide sequences. A PCR product with 502 pair bases in length was obtained for all specimens and subjected to digestion by eight restriction enzymes Alui, Tacit, MO, Rsal, Hinalli, Dral, Prull and Mien DNA banding, patterns in all specimens were similar and no polymorphism was detected among them. We conclude that cuttlefish populations cannot be isolated using 18s rRNA gene extracts in the area of study.en_US
dc.language.isofaen_US
dc.relation.urihttp://isfj.areo.ir/en_US
dc.subject.otherCuttlefishen_US
dc.subject.otherSepia pharaonisen_US
dc.titleGene 18s rRNA variation of cuttlefish population (Sepia pharaonis) in the Persian Gulf and the Oman Sea using PCR-RFLP methoden_US
dc.typeJournal Contributionen_US
dc.bibliographicCitation.issue2en_US
dc.bibliographicCitation.titleIranian Scientific Fisheries Journalen_US
dc.bibliographicCitation.volume14en_US
dc.description.statusPublisheden_US
dc.format.pagerangepp.157-168en_US
dc.subject.asfaPCR-RFLPen_US
dc.subject.asfarRNAen_US
dc.subject.asfaPopulationsen_US
dc.type.refereedRefereeden_US
refterms.dateFOA2021-01-30T18:48:29Z


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