17, 20 α Dihydroxy Progesterone (17, 20 α DP) production by different tissue in Common Carp (Cyprinus carpio) and Gold Fish (Carassius auratus)

Abstract

Different tissue (muscle, heart, eye, testis and blood) and sperm from Common carp and Gold fish, in order to evaluate activity of 20α hydroxy steroid Dihydrogenase (20α HSD) enzyme to convert radioactive 17α HP and amount of 0.01, 1 or 10 mg of nonradioactive hormones, were studied. Converting of substra to 17, 20α DHP was more than 30% in 100mg of eye, heart and testis tissue, 12% in 20cc blood and 18% in 20cc sperm, but it was less than 5% in 100 mg of muscle tissue. 17, 20α DHP was the only metabolite in non gonadal tissues incubations no significant enzyme activity was found in lense-eyeball fluid and retin of common carp. Possible relationship between 20α and 20β dihydroxy steroid dehydrogenase enzymes in fish, 20 hydroxy steroid dehydrogenase and aldo and Keto reductase enzymes has been discussed.