Comparison study on different synthetic hormones used for artificial propagation of Stellate Sturgeon Acipenser stellatus stellatus Berg, 1932

Abstract

In this study the effects of LHRHa, a mixture or common carp and sturgeon pituitary (CCP +SPE), Thyroxin hormone (T3) with commonly method used in hatcheries were compared. The experiments were conducted using different doses of hormones with several replicates in order 10 determine the most effective dose. Total number of broodstocks were females and 22 males. Prior to injecting the fishes, the Germinal Vesicle (GV) situation of all broodstocks were determined. The results showed that bloodstocks has been injected with LHRHa were very satisfactory and the percentage of response, fertilization and hatching of them had significantly different. The least effective dose of LHRHa that induced maturation was single injection and 350-375µg/fish for double injection. Thus it was determined that under the experimental condition, 20.0 to 31.2 µg/Kg of body weight of broodstock was the most effective dose. In the case of male broodstocks LHRHa was the most effective hormone and the sperms collected from these broodstocks were better in quality and quantity. The most effective dose of this hormone for males was determined to be 200 µg/fish or 27.7 to 31.2 µg/kg of body weight. The best dose of mixture of CCP + SPE which all broodstocks responded was7.0mg/kgBW +2.5mg/kgBW for females and 7.0mg/kgBW+ 1.5mg/kgBW for males. In the case of broodstocks that were first injected thyroxin and latter injected SPE it must be stated that since the thyroxin used was edible grade none of the broodstocks attained maturity. Therefore the results obtained in this treatment were not satisfactory.