Genetic variation of Barbus Capito in the southern Caspian Sea by PCR-RFLP method

Abstract

In this study 60 samples were collected from the southern Caspian Sea and some rivers of Mazandaran and Guilan provinces. Genetic variation and probable population differentiation of Barbus capito were studied based on the mitochondrial cytochrom-b gene. The mitochondrial DNA was extracted from fish fin using phenol-chlorophorm method. The specific primers were designed for B. capito and the PCR experiments were done on 60 samples. 11 restriction endonuclease enzymes were applied for RFLP analysis (ALuI, AvaI, AvaII, HinfI, DdeI, HincI, HpaII, RsaI, Sau3AI, HaeIII, TaqI). PCR products (1062 pb) and DNA digests were subjected to agarose and polyacrilamid gel electrophoresis to seperate fragments according to their molecular weight. These patterns were identified similar fpr all samples. Regarding to this patterns, it can be seen that polymorphysm phenomena cannot be observed by above mentioned enzymes and cytochrome-b gene, and there is no seperate population of B. capito in the southern Caspian Sea.