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dc.contributor.authorLalouei, F.
dc.contributor.authorRezvani Gilkolaei, S.
dc.contributor.authorPourkazemi, M.
dc.coverage.spatialIranen_US
dc.coverage.spatialSouthern Caspian Seaen_US
dc.coverage.spatialMazandaran Provinceen_US
dc.coverage.spatialGuilan Provinceen_US
dc.date.accessioned2018-05-22T11:42:49Z
dc.date.available2018-05-22T11:42:49Z
dc.date.issued2003
dc.identifier.issn1026-1354
dc.identifier.urihttp://hdl.handle.net/1834/12949
dc.description.abstractIn this study 60 samples were collected from the southern Caspian Sea and some rivers of Mazandaran and Guilan provinces. Genetic variation and probable population differentiation of Barbus capito were studied based on the mitochondrial cytochrom-b gene. The mitochondrial DNA was extracted from fish fin using phenol-chlorophorm method. The specific primers were designed for B. capito and the PCR experiments were done on 60 samples. 11 restriction endonuclease enzymes were applied for RFLP analysis (ALuI, AvaI, AvaII, HinfI, DdeI, HincI, HpaII, RsaI, Sau3AI, HaeIII, TaqI). PCR products (1062 pb) and DNA digests were subjected to agarose and polyacrilamid gel electrophoresis to seperate fragments according to their molecular weight. These patterns were identified similar fpr all samples. Regarding to this patterns, it can be seen that polymorphysm phenomena cannot be observed by above mentioned enzymes and cytochrome-b gene, and there is no seperate population of B. capito in the southern Caspian Sea.en_US
dc.language.isofaen_US
dc.relation.urihttp://isfj.areo.ir/en_US
dc.titleGenetic variation of Barbus Capito in the southern Caspian Sea by PCR-RFLP methoden_US
dc.typeJournal Contributionen_US
dc.bibliographicCitation.issue1en_US
dc.bibliographicCitation.titleIranian Scientific Fisheries Journalen_US
dc.bibliographicCitation.volume12en_US
dc.description.statusPublisheden_US
dc.format.pagerangepp.117-130en_US
dc.subject.asfamtDNAen_US
dc.subject.asfaRFLPen_US
dc.subject.asfaPCRen_US
dc.subject.asfaBarbus capitoen_US
dc.subject.asfaGenetic variationen_US
dc.subject.asfaSamplesen_US
dc.subject.asfaMitochondrial cytochrom-ben_US
dc.subject.asfaDNAen_US
dc.subject.asfaPhenol-chlorophormen_US
dc.subject.asfaALuIen_US
dc.subject.asfaAvaIen_US
dc.subject.asfaAvaIIen_US
dc.subject.asfaHinfIen_US
dc.subject.asfaDdeIen_US
dc.subject.asfaB. capitoen_US
dc.type.refereedRefereeden_US
refterms.dateFOA2021-01-30T18:48:24Z


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