Investigation on Salmo trutta caspius intestinal variations on different levels of dietary nucleotide

Abstract

The effect of specific nutrients on Salmo trutta caspius intestinal variation are practically unknown. The purpose of this work was to study the effects of different levels (0, 0.25 and 0.5%) of dietary nucleotide that added in control meal, on several segment of intestinal structure of Caspian salmon juvenile with average001/0± 26/12 g weight (average length 11/1±0/002). This trial was achieved in fiber glass 300 liter fresh water tank with 35 fish in each one. Fish cultured in 8 weeks and experiment has done on fish with average 26 g weight. 6 fish from each treatment captured, sacrificed and immediately gastro intestinal tract fixed by immersion into Bouin solution for histology. Then histological sections of 4 mm obtained, stained with Hematoxiline-Fushin and investigated. Results indicate that in duodenum nucleotide has a significantly (P<0.05) effects on villi length, thickness of muscle and sub mucosa. This treatment have been showed maximum of villi length and tickness of muscle. Similar results have been showed in ileum and jejunum. In this segments nucleotide have a significantly (P<0.05) effects on maturation and growth of villi length, muscle and submucosa.