Assay of Genetic diversity of cuttlefish (Sepia pharaonis; Ehrenberg, 1831) populations using microsatellite markers in Bandarabass and Bushehr regions

Abstract

In this present study genetic diversity of cuttlefish (Sepia pharaonis) populations were investigated using microsatellite markers. Total 51 samples were collected from Bandarabass and Bushehr regions. Tissue sample of arm tips (tentacle) were preserved in 96% ethanol alcohol until using in biotechnology laboratory of Khorramshahr University of Marine Science and Technology. Genomic DNA was extracted with CTAB method. The quality and quantity of extracted DNA was assessed by 1% agarose gel electrophoresis and spectrophotometry, respectively. Polymerase chain reaction conducted with 6 pairs of microsatellite primers. PCR products were electrophoresed on 8% polyacrylamide gel and stained with silver nitrate. These primers were shown 4 pairs of polymorph and 2 pairs of monomorp. Allele Sizes were measured in populations then genetic parameter were calculated using Arlequin and Gen Alex Programs and phylogenetic relationship was determinated and drawn using TFPGA Program. Result obtained showed genetic distance and resemblance distance is 0.282 and 0.754, respectively and Weak but significant genetic differentiation was present 0.031 between of populations.