Identification of WSSV,TSV & IHHNV in wild and cultured shrimps and crustaceans in khuzestan province - Abadan and investigation of its pathogenicity

Abstract

According to the white spot disease during 2002 until 2005 and the damages caused by the disease to the farmers a new species L.vannamei were introduced to the farming region of Abadan Choebdeh from 2006. Hight mortality occurred in Khuzestan province farms in 2007 and 2008 again. Probably viral agent was transferred to native shrimps or other aquatic animals, therefore it is very important that presence of viruses in local aquatic animals and its vectors detected. Detection of WSSV, TSVand IHHNV in aquatic animal from Khuzestan costal region imphasis to wild shrimp and craps is the main objectives of this study. So samples were taken from 10 active farms twice a week and 100 postlarves from 3 active breeding center befor stocking.Samples for viral detection were studied by molecular and histopathology asseys. Results was shown presence of the White spot virus in cultured and wild shrimp and TSV infection in only two cultured shrimps but there was negative result for IHHN virus. To study the pathogenesis of white spot virus isolated from wild and farmed shrimps, exposure method was used. Results of challenge showed that WSSV can be transmited from diseased cultured and wild shrimp to cultured shrimp. Mean mortality percent in the treatment that fed with infected wild shrimp was 38.33± 10.4 and treatment that fed with infected cultured shrimp 61.66 ± 7.63 and in the positive control treatment was 87.5 ±3.5.