Isolation, Identification and Cloning of growth hormone gene from Epinephelus coioides in E. coli
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Date
2016
Metadata
Show full item recordAbstract
According to the role of the growth hormone gene in the aquaculture, in this research cloning growth hormone coioides (Epinephelus coioides) was evaluated in vector pTZ57R / T. After PCR product purification by kit QIAquick Gel Extraction, growth hormone gene was integrated into pTZ57R / T vector. Constructed vector was transferred in E. coli strain DH5α competent cells. The white colonies was recombinant bacteria, from that that plasmid extraction was performed. Cloning obtained conformed using with direct PCR and sequencing methods. Growth hormone cDNA from E. coioide has an open reading frame of 615 nucleotides and 204 amino acids respectively. The calculated molecular weight and predicted of isoelectric point of the growth hormone protein were 014/23 kDa and 9.6 respectively. The results of this study showe that growth hormone gene in pTZ57R / T vector successfully had been cloned and can be used to growth hormone gene expression and protein production. The omparition of gene sequencing with growth hormone gene from E. coioides in the GenBank was showed similarity between them .Journal
Journal of Marine Science and TechnologyVolume
14Issue/Article Nr
4Page Range
pp.108-117Collections