Genetic diversity of Crassostrea genus using 16S rRNA from coastal of Emam Khomayni Port

Abstract

Nucleotide sequence polymorphism associated with 16S rRNA gene of mitochondrial genome of the Persian Gulf Oyster (Crassostrea sp.), examined in 30 samples collected from Bandar-e Emam Khomeini. Polymerase chain reaction was performed by a pair of primer for all samples and PCR products were sequenced using dideoxy chain termination method . Then the obtained data were analyzed usig MEGA Ver 5.0, BioEdit Ver 7.0 and DnaSP Ver 5.0 programs and software. The results showed 2 mutation and Nucleotide diversity (Pi) 0.00061 observed in studied genes. Among the 30 samples, 3 haplotypes were detected and average haplotype diversity (Hd) 0.195 were calculated. The haplotypes obtained in this study were submitted in the Gene Bank for the first time. Phylogeny trees derived from 16S rRNA gene sequencing showed no significant separation of the samples in the Imam Khomeini port. The results of this study also showed that there is low sequence differentiation in the study population in Imam Khomeini port.