Effects of different levels of vitamin C on hatching efficiency and survival of Maharloo Lake Artemia nauplii (Artemia parthenogenetica) at different temperatures

Abstract

In this study, different levels of vitamin C (L-ascorbyl-2-polyphosphate) on the hatching efficiency and obtained Artemia nauplii survival were examined at different temperatures. In the first experiment, the effect of different amounts of vitamin C (0, 500 and 1000 ppm) on hatching percentage of the Maharloo Lake Artemia cyst was examined with 6 replicates. Results of the first experiment showed that different amounts of vitamin C had significant effect on hatching percentage. In the second experiment, the effect of different temperatures on the percentage of survival of Artemia nauplii was studied. In this experiment, obtained nauplii from first experiment were put in 3 different temperatures (1, 4 and 16ºC) with 6 replicates for 48 hours, then percentage of survival of Artemia nauplii was determined. Result of the second experiment showed that interaction of vitamin C and different temperatures had no significant effect on the survival rate of Artemia nauplii. Different amounts of vitamin C had no significant difference on the percentage of survival of Artemia nauplii, but different temperatures had significant effect on survival rate. Results of these experiments showed that increase of the amount of vitamin C had significant difference on hatching percentage of the Maharloo Artemia cyst, but no significant effect on survival rate of Artemia nauplii during holding at different temperatures. Also it is found that survival rate of Artemia nauplii at 16ºC temperatures was more than 1 and 4ºC temperatures. The results showed independent of the amount of vitamin C, holding temperature of 16ºC could be suitable compared with the other examined temperatures.