Introducing standard protocol for enrichment of Artemia urmiana nauplii with Canola oil

Abstract

This research was performed to introduce a standard protocol for enrichment of Artemia urmian with Canola oil. Artemia urmiana nauplii were enriched at three densities (50000, 100000 and 200000 nauplii L -1 ) and three concentrations of Canola oil (0.1, 0.2 and 0.3 g L1 ). Their effects were evaluated on survival, total length and profile of fatty acids at 6, 9, 12, 15 and 18 hours after the onset of enrichment. Cysts of A.urmiana were hatched according to the standard method. A.urmiana nauplii were stocked at above densities in 7 L cylindrical containers. Canola oil emulsion was added at concentrations of 0.1, 0.2 and 0.3 g L-1 at the beginning and 12 hours after the onset of enrichment. The results of analysis showed that enrichment of A.urmiana with 0.3 g L -1 Canola oil at 100000 nauplii L-1 for 18 hours was considered as the best treatment. Artemia nauplii enriched in this treatment had significantly higher levels of (n-3) PUFA and survival and minimum total length comparing to other treatments. The treatment had significantly higher levels of (n-6) PUFA than all treatments except treatment with a density of 50,000 nauplii L-1 with 0.1 g L -1 Canola oil for 18 hours.