Genetic characterization of Sillago sp. from Hormozgan coastal waters using microsatellite markers

Abstract

Using 5 microsatellite loci Sillago sp. From Hormozgan North Persian Gulf were investigated through 68 specimens in two stations: Minab(31 specimens) and Bandar Lengeh(38 specimens). DNA was extracted using modified CTAB(Hexadecyl trimethyl-ammonium bromide) protocol. 5-17 alleles were observed in 5 loci with 8.4 mean allelic frequency. Observed and expected heterozygosity values was calculated (0.115<Ho<0.792) ,(0.598<He<0.902) . Genetic distance and identity according to Nei was estimated 0.520 and 0.595 respectively which shows genetic distance of genus level. Significant deviation from Hardy-Weinberg equilibrium (p<0.001) was observed at all loci at two stations. Analysis of Molecular Variance(AMOVA) of Genetic differentiation index(Fst) revealed mean significant (p=0.001) differentiation between two studied regions and 3.90 gen flow. Despite low distance of two studied regions (180km) there are probably two distinct populations of Sillago sp. which should be considered in management efforts.