Genetic characterization of Sillago sp. from Hormozgan coastal waters using microsatellite markers
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Author
Shadi, AhmadZolgharnein, Hosein
Salari-aliabadi, Mohamad Ali
Nabavi, S.M.B.
Ronagh, Mohamad Taghi
Date
2014
Metadatos
Mostrar el registro completo del ítemAbstract
Using 5 microsatellite loci Sillago sp. From Hormozgan North Persian Gulf were investigated through 68 specimens in two stations: Minab(31 specimens) and Bandar Lengeh(38 specimens). DNA was extracted using modified CTAB(Hexadecyl trimethyl-ammonium bromide) protocol. 5-17 alleles were observed in 5 loci with 8.4 mean allelic frequency. Observed and expected heterozygosity values was calculated (0.115<Ho<0.792) ,(0.598<He<0.902) . Genetic distance and identity according to Nei was estimated 0.520 and 0.595 respectively which shows genetic distance of genus level. Significant deviation from Hardy-Weinberg equilibrium (p<0.001) was observed at all loci at two stations. Analysis of Molecular Variance(AMOVA) of Genetic differentiation index(Fst) revealed mean significant (p=0.001) differentiation between two studied regions and 3.90 gen flow. Despite low distance of two studied regions (180km) there are probably two distinct populations of Sillago sp. which should be considered in management efforts.Journal
Journal of Marine Science and TechnologyVolume
13Issue/Article Nr
2Page Range
pp.1-10Colecciones