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dc.contributor.authorMirbakhsh, Maryam
dc.contributor.authorZorriehzahra, S.J.
dc.contributor.authorYeganeh, V.
dc.contributor.authorGhaednia, B.
dc.contributor.authorDashtiannasab, A.
dc.contributor.authorMohammadi Baghmollai, E.
dc.contributor.authorMehrabi, M.R.
dc.contributor.authorNazari, M.A.
dc.contributor.authorSamani, N.
dc.contributor.authorParsa yeganeh, L.
dc.contributor.authorAfsharnasab, M.
dc.contributor.authorKeshtkar, I.
dc.coverage.spatialIranen_US
dc.date.accessioned2018-08-09T07:23:05Z
dc.date.available2018-08-09T07:23:05Z
dc.date.issued2016
dc.identifier.urihttp://hdl.handle.net/1834/13892
dc.description.abstractAt present, the aquaculture industry to provide proper instructions in the field of health management, including production of Specific Pathogen Free shrimp (SPF), require sensitive and reliable methods for the detection and identification of pathogenic microorganisms. Molecular methods which used in the detection of microorganisms have a high discriminatory power in the taxonomy and in relation to libraries in the world. On the other hand, the accurate identification of microorganisms, providing the genetic data bank of shrimp pathogens and maintenance of these strains is the step to promote further research on the mechanisms of pathogenesis of pathogens, diagnosis, treatment, prevention of disease, identify indigenous production kits, diagnosis re emerging and emerging diseases and their origin. Therefore, in this project, by using ribotyping technique, native isolated pathogenic bacteria and fungi were identified and recorded in the gene bank database center. During sampling of shrimp and water of Specific Pathogen Free shrimp center, 40 bacterial strains were isolated, which 8 of them had the most frequency and identification based on 16S rDNA sequencing was performed. Bacteria identified are: Vibrio nigripulchritudo strain IS013(GenBank:KP843725), Vibrio brasiliensis strain IS014 (GenBank:KR186076), Vibrio rotiferianus strain IS015 (GenBank:KR186077), Vibrio azureus strain IS012 (GenBank:KJ018724.1), Vibrio owensii strain IS016 (GenBank:KR186078), Agarivorans gilvus strain IS017 (GenBank:KR186079), Vibrio brasiliensis IS018 (GenBank:KR186080) and Vibrio alginolyticus strain IS019 (GenBank:1817854), which were recorded in The World Bank genes. In this study fungal isolates were not detected.en_US
dc.description.sponsorshipIranian Fisheries Science Research Instituteen_US
dc.language.isofaen_US
dc.publisherIranian Fisheries Science Research Instituteen_US
dc.relation.ispartofseries50711;
dc.titleMolecular identification of bacterial and fungal pathogens in certain disease-free shrimpen_US
dc.typeReporten_US
dc.description.statusPublisheden_US
dc.format.pages64pp.en_US
dc.publisher.placeTehran, Iranen_US
dc.subject.asfaBacteriaen_US
dc.subject.asfaFungien_US
dc.subject.asfaRibotypingen_US
dc.subject.asfa16S rDNAen_US
dc.subject.asfaShrimpen_US
dc.subject.asfaSpecific pathogen freeen_US
dc.subject.asfaVibrio rotiferianusen_US
dc.subject.asfaVibrio owensiien_US
dc.subject.asfaVibrio brasiliensisen_US
dc.subject.asfaVibrio azureusen_US
dc.subject.asfaAgarivorans gilvusen_US
dc.subject.asfaVibrio alginolyticusen_US
dc.subject.asfaGenBanken_US
dc.type.refereedRefereeden_US
refterms.dateFOA2021-01-30T18:48:33Z


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