A study on methanol herbal plant extract of Garlic (Camellia sinensis) on immunity level of Mugil cephalus

Abstract

The aim of this study was to investigate the effect of green tea on a non-specific immunity of Mugil cephalus. Three hundred and sixty Gray mullet larvae with average weight of 0.75 ± 0.02 g and an average length of 4.40 ±0.81cm collected from Ramin port where is located at 5 km far from the Chabahar and finally transferred to lab of trial in Offshore Research Center. This research was designed based on 3 treatments, each with 3 replicates. The major factors examined containing growth factors mainly consists of average weight, average daily gain, protein efficiency rate, protein productive rate, hematological and biochemical factors including globin, albumin, total protein, lysozyme, ultimately, immunological factors, and respiratory burst and phagocytosis percent . In general, the results of this study showed that the addition of 200 mg of green tea extract per kilogram of diet led to a significant increase in final weight, food intake, daily growth rate and efficiency of protein gray mullet were compared to control. Fish fed diet 50 to 200 ppm green tea extract, showed a significant increase in the number of erythrocytes to control. The number of white blood cells from control with 200 ppm of green tea extract regularly gradually increased. The highest amounts of hemoglobin in the samples containing 200 ppm of green tea extract were measured. The hematocrit in control and the highest levels in samples with 200 ppm of green tea extract were measured. The total protein, albumin and globin in the blood serum fed treatments with green tea extract showed a significant increase compared to the control treatment (p< 0.05). The highest total protein, albumin and globin treatment with 200 ppm of green tea extract of food was observed (p< 0.05). The highest activity of lysozyme ,phagocytosis respiratory burst were observed in treatment green tea extract 200 ppm of food. Treatment with 200 ppm of food. showed a minimal mortality after challenge with bacteria photobacterium damselae compared to the control treatment.