The comparative study of the streptococcinum, Hepar sulfur, Rosmarinus officinalis and erythromycin effects on cultured rainbow trout (Oncorhynchus mykiss) with experimental streptococcusis.

Abstract

The comparative study of the streptococcinum, Hepar sulfur, Rosmarinus officinalis and erythromycin effects on cultured rainbow trout (Oncorhynchus mykiss) with experimental streptococcosis Homeopathy is one of alternative medicines that is very useful for soul and body diseases with accurate prescription . The goal of this study was “ survey of the effects of homeopathic remedies. There is not any more research about homeopathy on aquatics in the world especially in Iran, thus Some research about the effects of homeopathy on aquatics is needed. In this study , the effects of streptococcinum,Hepar sulfure, Rosmarinus officinalis(homeopathic remedies) and erythromycin in cultured rainbow trout ,with experimental streptococcosis, also the mortality, were compared. There was 6 treatment and 2 reviews in 300 liter tanks that each of treatment contained 40 juvenile rainbow trout with 25±5 gr arrange weight . Treatment 1: contained of erythromycin. Treatment 2: Streptococcinum C30. Treatment 3: Rosmarinus officinalis Q. Treatment 4: Hepar sulfur C30 . Treatment 5:(control treatment )without any injection and any therapy . Treatment 6 : ( positive control treatment ) with injection but without any therapy. Daily estimation of the water temperature , oxygen, PH and salinity and some other chemical factors. Tretment 1 had significance with the other treatments. Survival percent in treatments and their analysis showed that treatment 4(Hep-s) had significance (p<0.05) with treatment 3(Ros-off) and its survival percent is more than the other homeopathic remedies. Erythromycin is chemical drug and has many side effects but Hep-s has not any side effect and is an natural remedy for Streptococcosis in homeopathy. Thus we offer the Hep-s to cure the streptococcosis but some research with disk diffusion test about the different doses of Hep-s is needed. Daily survey of clinical symptoms such as hemorrhages in the external organs, Under eyes, under skin, under fins and gills, hemorrhages and exophthalmos , were the most symptoms . Important pathological symptoms were: necrosis , hyperplasia and melanosis in branches, liver and kidneys, hemorrhages in heart, kidneys and in visceral tissues. According to the survival results, there was significant difference between the treatment 1 and the other treatments(p<0.05). Also there was significant difference between treatment 3, treatment 4 and treatment 2(p<0.05), this difference is due to the high dose(Q) of R.officinalis, while the two other homeopathic remedies were in a moderated dose (C30), 30× 100 diluted dose. Survival percent of treatment 4(Hep-s) was more than the other homeopathic remedies and was related to erythromycin. Erythromycin is chemical medicine and has many side effects, while Hepar sulfur has not any side effects if the prescription would be accurate. Hep-s is suggested with disk diffusion tests for relief the symptoms of streptococcosis.