The Study on health situation of shrimp Litopenaus vannamei broodstoock production in earthen pond and comparing with fiberglass or cement tanks

Abstract

The investigation of health and diseas situation of shrimp broodstook (Litopenaeus vannamei) in Boushehr province in earthen pond and cancreat and fiberglas was carried out from May 2014 until July 2014 with collected 100 samples brood stook from earthen pond and 100 samples from earth and fiberglas thank.The clinical sign of samples documented in take history form and then the samples transport to Iran Shrimp Research Instituet in Bousheher. The bacterial and fungal studied was carried out with hemolymp, hepatopacreas and gill tissue and then the shrimp preserved in Davidson Fixative for histopathology. A part of uropoda also preserved in ethyl alcholo for PCR study and detecting viruses. The result showed 10 bacteria consist V.harveyii, V.parahaemolyticus, V.anguillarum, V.vulnificus, V.mimicus, V.damsela,V.nereis, plesiomonas shigelloides V.alginolyticus V.proteolyticus, in earthen pond and 5 bacteria consit ,V.alginolyticus V.proteolyticus ، V.parahaemolyticus ، V. damsela ،V.mimicus were identified in fiberglas thank. In this study 10 fungi consist Penecilium, Asp. Niger, Asp. Flavious, Asp. fomigatus, Acromonium, Ulocladium, Mucor, Cladosporium, Alternaria, Rhizopus, and 5 of them were identified in both broodstock from earthen pond and fiberglas thank. However 6 parasite consist of zoothamnium, vorticella, Acneta, Ephelota, Epistylis, Epistylis and Apastomom identified in earthen pond and three of them were identified in fiberglas thank. In histoplatholgy some tissue showed the effect of vibrio infecting in different organs as well in gill and midgut and the PCR examined were negative for all viruses. Regarding the produce healthy broodstock we need excuted the High Health procdure.