The survey and identification source of white spot disease in shrimp Farm in Abadan area

Abstract

In the summer of 2002 high mortality occurred in farm shrimp in Abadan area due to white spot disease. The main objective of this study was detection and identification the source of white spot syndrome virus. For this reason from 2004/11/4 until 2005/120/6 about 190 samples consisted of wild shrimp, crab, fish, and plankton collected and preserved in alcohol 90-75% for polymerase chain reaction (PCR).The samples transferred to the South Iranian Aquaculture Center in Ahwaz. The samples examined by commercial WSSV detection kit (Fast Target) and new kit design by Iranian Fisheries Research Organization (IFRO) and Biotechnology Research Center from Iran. The result from PCR showed the samples of Penaeus indicus brood stock and postlarvea, Parapenaeopsis stylifrus and Metapenaeus affinis was positive. The rest of samples did not show any sign of virus and they was negative by PCR. Because the disease outbreak occurred in 2002 and the samples collected in 2004, the situation of environmental in the area was changed and may be the virus disappears in the samples and died.