Increasing growth rate of Salmo trutta caspius by selection (Phase 1)

Abstract

The management of genetically brood stocks of Salmo trutta of Caspian Sea in order to restocking for longe time harvesting and culture of fish in intensive system, has been little investigated, in contrast with European stocks. Salmo trutta Caspius is very important in biological and genetically point of view. This fish as gene bank valid for breeding improvement, hybridization and new cold species for culture, can be used. This study begain in 2007 and lasted 3 years in Bahonar, kelardasht of Iran. The breeders were 27 pairs. Each female have been crossed by 3 male. Eggs and larvae as well as the fingerlings reared separately of each crosses. The best fingerlings of 5, 20 and 100 gram were collected after 3 period of challenge. At the final 300 brood stocks in two groups were remained and stocked for artificial propagation. For heritability estimation in 4 stages sampling the weight and length were measured and it was 0.47 and 0.50 respectively. In case of molecular study the quality of DNA extract from the fin of breeders and their progeny by phenol-chloroform was good for PCR test. The heterosigosity test for Ho and He for each station and sites except for SRUTTA12 and STRUTTA59, the He was greater than Ho. Shaton Index and Hardy-Weinberg equilibrium for station and sites for male and progenies, the OM24showed deviation from H-W equilibrium. In case of similarity and genetic distance based on Nei (1972), the highest distance were in case of male and female, and were the least between fingerlings and male brood stocks.