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dc.contributor.authorNoruzi Moghadam, H.
dc.coverage.spatialIranen_US
dc.date.accessioned2018-08-27T07:22:46Z
dc.date.available2018-08-27T07:22:46Z
dc.date.issued2003
dc.identifier.urihttp://hdl.handle.net/1834/14158
dc.description.abstractAnnually, increasing antropogenic impact on environment results in a considerable aggravation of the ecological condition and extinction of many species of organisms and a decrease in the abundance of Acipenseridae fish family in the Caspian Sea. Therefore cryopreservation of the sturgeons sperm has become on urgent problem. Thus cryopreservation of gametes and embryos is considered to be one of the ways to preserve gene pool of the fish. In this research the broodfish originated from Turkman and Tazabad fish capture station in south of Caspian Sea. They were stored in fish breeding pond with river water flow for one week in shahyd Marjani and shahyd Rajaei fish farms. The males of broodfish were injected intramuscularly with action dryed sturgeon pituitary at doses of 2.5 mgkg-1 body weight. The sperm was collected 12h later, by a sound connected to 50ml syringe. Activity of fresh sperm wa determined by mean of microscope after its dilution by water. Then the fresh sperm was diluted by cryoprotective medium in proportion of 1:10 containing 0.1 M of Tris-HCl buffer, pH 8.6, 2% dimethyl sulphoxide, 25% yolk of chiken egg and 0.04 M saccharrose. sperm diluted using this way was poured into 1ml syringe and frozen according to two method: immediately or gradually. After 12h to 205h activity of frozen-thawed sperm was determined and used for fertilization. As the result showed that the percentage of foucoundation (success of fertilization) was 27 and the percentage of yolk sac embryo stage was 16.en_US
dc.description.sponsorshipIranian Fisheries Science Research Instituteen_US
dc.language.isofaen_US
dc.publisherIranian Fisheries Science Research Instituteen_US
dc.relation.ispartofseriesWithout No 7;
dc.titleThe possibility study of preservation and freezing sperm of two sturgeon species: Acipenser persicus, Acipenser stellatusen_US
dc.typeReporten_US
dc.description.statusPublisheden_US
dc.format.pages35pp.en_US
dc.publisher.placeTehran, Iranen_US
dc.subject.asfaSturgeonen_US
dc.subject.asfaSpermen_US
dc.subject.asfaFishen_US
dc.subject.asfaCryopreservationen_US
dc.subject.asfaAcipenser stellatusen_US
dc.subject.asfaAcipenser persicusen_US
dc.subject.asfaSpeciesen_US
dc.type.refereedRefereeden_US
refterms.dateFOA2021-01-30T18:48:35Z


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