Genetic Variation Survey of Sepia pharaonis in The Persian Gulf and Oman Sea Using Marker of RFLP

Abstract

This research was conducted to identify Cuttlefish population (Sepia pharaonis) in The Persian Gulf and the Oman Sea using PCR-RFLP. Specimens were collected from 15 different stations. Bottom trawling method was used for sampling from different zones of the Persian Gulf and the Oman Sea, and finally 20-40 specimens from S.Pharaonis were collected at each station . DNA was extracted by phenol chloroform method. One pair primer was designed based on 18s rRNA gene nucleotide sequences. The results obtained from 18s rRNA gene RFLP, which was reproduced by PCR technique, were analyzed and utilized for study of diversity of the Cuttlefish population . PCR product with 502 pair base in length achieved for all specimens, which was subjected to enzymatic digestion by 8 restriction action enzymes: Alu I-Taq I-Mnl I- Rsa I-Hind III-Dra I-Pvu II and Hae II DNA bands patterns in all specimens digested by those enzymen showed similarity with no any polymorphism. From this result, it can be concluded that there is not any possibility to isolate different populations in the studied Cuttlefish species under exploitation of rRNA gene.