Show simple item record

dc.contributor.authorMohammadrezaei, D.
dc.coverage.spatialIranen_US
dc.date.accessioned2018-10-05T12:25:29Z
dc.date.available2018-10-05T12:25:29Z
dc.date.issued2018
dc.identifier.issn1026-1354
dc.identifier.urihttp://hdl.handle.net/1834/14642
dc.description.abstractThe vitellogenin gene expression can be altered by some estrogenic plant compounds like Genistein and β-sitosterol. Therefore, the measurement of vitellogenin gene expression can be used as an indicator to determine their effect on reproductive performance of aquatic animals. In order to evaluate the effects of genistein and β-sitosterol on the expression of vitellogenin gene in the liver and ovary of Rutilus frisii Kutum, the fish were separately exposed to 3 levels of genistein and beta-sitosterol (500, 50 and 10 ng/L). After 21 days, the RNA extracted and expression of vitellogenin gene in both the liver and ovary was investigated by Real-time PCR. The results showed the level of vitellogenin gene expression in fish exposed to genistein was higher in liver than control and β-sitosterol treatment. This difference was not observed in the ovarian tissue. Because the main site of vitellogenin synthesis expressed liver and it was controlled by endogenous estrogen (E2), so, it seems phytoesterogenic compound such as genistein has been able to increase the relative expression of this gene in fish Exposed to 500 ng/L.en_US
dc.language.isofaen_US
dc.relation.urihttp://isfj.areo.ir/en_US
dc.titleIdentification of vitellogenin gene expression patterns in liver and ovary of Rutilus frisii Kutum exposed to genistein and β-sitosterolen_US
dc.typeJournal Contributionen_US
dc.bibliographicCitation.issue3en_US
dc.bibliographicCitation.titleIranian Scientific Fisheries Journalen_US
dc.bibliographicCitation.volume27en_US
dc.description.statusPublisheden_US
dc.format.pagerangepp.153-158en_US
dc.subject.asfaGenisteinen_US
dc.subject.asfaβ-sitosterolen_US
dc.subject.asfaVitellogeninen_US
dc.subject.asfaGene expressionen_US
dc.subject.asfaRutilus frisii Kutumen_US
dc.subject.asfaIdentificationen_US
dc.subject.asfaOvaryen_US
dc.subject.asfaLiveren_US
dc.subject.asfaGeneen_US
dc.subject.asfaRNAen_US
dc.subject.asfaPCRen_US
dc.subject.asfaFishen_US
dc.type.refereedRefereeden_US
refterms.dateFOA2021-01-30T18:48:27Z


Files in this item

Thumbnail
Name:
ISFJ-v27n3p153-en.pdf
Size:
545.6Kb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record