• English
    • français
    • español
    • русский
  • English 
    • English
    • français
    • español
    • русский
  • Login
View Item 
  •   Home
  • ASIA
  • Iran (Islamic Republic of)
  • Islamic Azad University, Science and Research Branch, Tehran
  • Islamic Azad University, Science and Research Branch, Tehran. Publications
  • View Item
  •   Home
  • ASIA
  • Iran (Islamic Republic of)
  • Islamic Azad University, Science and Research Branch, Tehran
  • Islamic Azad University, Science and Research Branch, Tehran. Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Browse

All of AquaDocsCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

My Account

LoginRegister

User Information

About AquaDocsPoliciesUser GuidesOther Aquatic Repositories

Statistics

Display statistics

Effect of sub-accute exposure of Cu nanoparticles on histopatological, DNA damage and oxidative enzymes changes in ( Rutilus caspicus Yakovlev, 1870 )

  • CSV
  • RefMan
  • EndNote
  • BibTex
  • RefWorks
Thumbnail
Name:
رساله.pdf
Size:
5.836Mb
Format:
PDF
Download
Average rating
 
   votes
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item. When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
 
Your vote was cast
Thank you for your feedback
Author
Aghamirkarimi, Shahrzad
Mashinchian Moradi, Ali
Sharifpour, Issa
Jamili, Shahla
Ghavam Mostafavi, Pargol
Date
2017-09

Metadata
Show full item record
Abstract
The current study has determined the toxicity effects of copper nanoparticles on the some vital organs such as gill, liver and kidney of Caspian Roach; Rutillus caspicus. For this purpose, 120 fishes were used as experimental fishes and exposed to 0.1, 0.2 and 0.5 mg/L of Cu nanoparticles for 21 days, and 30 fishes as control. The mean water temperature of the aquaria was 22±2 ºC, dissolved oxygen 5.2 mg/L, pH at 7±0.004 and the concentration of calcium carbonate was 270 ppm. On the 7, 14 and 21 days after exposing the fishes to Cu nanoparticles, three fish were randomly selected from each aquaria, sacrificed and samples from their gill, liver and kidney were taken and fixed in cold 10 % buffered formalin. Then microscopic sections were prepared and examined by light microscope which showed histological alternations in the gill, liver and kidney tissues. Then blood samples collected for enzymatic and DNA damage evaluation. Histological al-ternation in gills included: hyperplasia, fusion and detachment of secondary lamellae, blood congestion in vascular axis of primary filaments, reduced secondary lamellae length and cellular degeneration. Histolog-ical changes in liver included blood congestion in the central veins, cytoplasmic vacuolation of the hepato-cytes, cellular degeneration, congestion in the blood sinusoids and necrosis of the hepatocytes. Histological changes in kidneys included glomerular shrinkage, severe degeneration in the tubules cells, interstitial tissue and glomerulus, increase in interstitial tissue cells and macrophages aggregation.The most severe alternation of the gill, liver and kidney were in third weeks at 0.5 mg/l concentration. There were positive correlation between concentration and hyperplasia (r=.815, p<.01), fusion (r=.620, p<.05), detachment (r=.849, p<.01), blood congestion (r=.706, p<.05), degeneration (r=.742, p<.01). There were positive correlation between time and telangiectasia (r=.611, p<.05). There were positive correlation between concentration and hepato-cytes hypertrophy (r=.652, p< .01), necrosis (r=.768, p<.05). The activity of antioxidant stress enzymes, superoxide dismutase (SOD) and catalase (CAT), were measured in the specimens. The maximum level of enzymes activities were in first week at 0.1 mg/l concentration. There were significant difference between treatments and control (p<0.05). We used comet assay for evaluation of DNA damage. The activity of anti-oxidant stress enzymes were higher in low concentration. DNA damage was significant in all treatments. The most severe DNA damage have seen in third week at 0.5 mg/l concentration. Evaluation of these changes could be useful in estimating the harmful effects of Copper nanoparticles. The degree of damages was more intensive at higher CuNP concentrations. The result of the study showed that CuNPs could cause severe damages on the Caspian roach; Rutillus caspicus and have lethal effects for fish.
Pages
113pp.
Degree
PhD
Publisher or University
Islamic Azad University, Science and Research Branch
URI
http://hdl.handle.net/1834/15434
Collections
Islamic Azad University, Science and Research Branch, Tehran. Publications

entitlement

 

DSpace software (copyright © 2002 - 2023)  DuraSpace
Quick Guide | Contact Us | Disclaimer
Open Repository is a service operated by 
Atmire NV
 

Export search results

The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.