Comparison of perfish solution efficiency with malachite green and formalin on fungal contamination control of rainbow trout (Oncorhynchus mykiss) eggs in incubation stage

Abstract

This research was conducted in autumn and winter 2016 in Shahid Motahary Coldwater Fishes Genetic and Breeding Research Center. In this study, comparison of Perfish solution efficiency with malachite green and formalin on fungal contamination control of rainbow trout (Oncorhynchus mykiss) eggs in incubation stage was done. For this porpuse the California incubators (20 × 35 × 30 cm) with depth of 20 cm, the height of water on the egg, 10 cm and water flow rate 4 to 6 liters per minute were used. Then the number of 700 fertilized eggs transported to each incubator tray. In this study, 9 treatments (perfish 15, 37, 75, 150, 300 ppm; malachite 2ppm; formalin 1000ppm; positive control and negative control) were tested with 3 replications. The highest Percentage of Eyed eggs in treatment of green malachite, formalin and perfish 75 ppm were observed respectively with 69.38±1.94, 64.99±8.05 and 63.76±4.72. The highest of hatching rate were observed in treatment of perfish 75 ppm (95.8±.6). Treatments of formalin and perfish 15 were showed the lowest (3.33±1.15) and highest (5.33±2.08) of larva deformity respectively, without the Significant difference among the experimental treatments. Finally the results showed that the best and more effective dose of Perfish was 75ppm in 30 min. furthermore with involving all aspects Perfish solution is a suitable new compound for the prevention of fungal contamination during the incubation period of Rainbow trout eyed eggs.