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dc.contributor.authorHasebi, Z.
dc.contributor.authorMotamedzadegan, A.
dc.contributor.authorMadani, R.
dc.contributor.authorZamani, A.
dc.coverage.spatialIranen_US
dc.coverage.spatialMazandaran provinceen_US
dc.date.accessioned2020-02-22T08:08:42Z
dc.date.available2020-02-22T08:08:42Z
dc.date.issued2020
dc.identifier.issn1026-1354
dc.identifier.doi10.22092/ISFJ.2019.119548
dc.identifier.urihttp://hdl.handle.net/1834/16479
dc.description.abstractIn recent decades, recovery and identification of digestive enzymes from fish has attracted much attention and new applications for these enzymes have been defined in some industries, such as the food and detergent industries. One of these recovered enzymes is Trypsin, which in this study was extracted and purified from the intestine of Caspian kutum using a three-step extraction method with 50 mM Tris-HCl buffer (containing 10 mM CaCl2 pH 7.5), 60% ammonium sulfate precipitation and Sephadex G-75 gel chromatography. The Result was an enzyme with 52% yield and a purity of 25.78-fold. The kinetic parameters of Vmax and Km for BAPNA substrate were calculated using the Michaelis-Menten equation and were 2.9958 µmol/mg.min and 0.5350 mM respectively and Kcat was 6.2568 S-1. The kutum Trypsin’s half-life was also calculated as 58.72 min for BAPNA substrate using the conversion of the enzyme activity graph (in minutes) to natural logarithmic graph and determining its slope. The results showed that it is easy and economical to extract and purify Trypsin from intestine of Caspian kutum and it is kinetically more effective than commercial Trypsin. It is suitable for use in the food industries, fish processing industry, culture media production as well as application in biotechnology research.en_US
dc.language.isofaen_US
dc.relation.urihttp://isfj.areo.ir/en_US
dc.subject.otherRutilus frisii Kutumen_US
dc.titleExtraction and purification of Trypsin from intestine of Caspian kutum (Rutilus frisii Kutum) and Calculation of its Kinetic parameters and Half Lifeen_US
dc.typeJournal Contributionen_US
dc.bibliographicCitation.issue5en_US
dc.bibliographicCitation.titleIranian Scientific Fisheries Journalen_US
dc.bibliographicCitation.volume28en_US
dc.description.statusPublisheden_US
dc.format.pagerangepp.101-110en_US
dc.subject.asfaFishen_US
dc.subject.asfaEnzymesen_US
dc.subject.asfaHalf Lifeen_US
dc.subject.asfaBiotechnologyen_US
dc.type.refereedRefereeden_US
refterms.dateFOA2021-01-30T18:48:29Z


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