• 17α-Hydroxy-4-pregenen-3-one (17 αP) assay, using acetylcholinesterase enzyme as tracer

      Ebrahimi, M. (2005)
      17a-hydroxy-4-pregnen-3-one (17aP) hormone is a precursor of other steroid hormones and radioimmunoassay has already been used to measure it, but a simple and rapid "Enzyme Linked Immunosorbant Assay" (ELISA) is described and validated here. A general procedure for prepara-tion of the acetylcholinesterase labelled steroid is described, which is applicable to any steroid. Use of acetylcholinesterase tracer increased the sensitivity of assay so that reliable measurements of each steroid could be achieved with only 10 ml of plasma. The ELISA was applied to measure 17, 20a-hydroxy-4-pregnen-3-one (17, 20aP) steroid production from 17aP by 20 a-hydroxy steroid dehydrogenase (20a-HSD) from sperm of roach (Rutilus rutilus). The results showed that cyprinid sperm contains potent and active 20a-HSD enzymes which produce 17, 20aP hormone from 17aP substrate.
    • A comparison on FCR in fish ponds of rainbow trout (Oncorhynchus mykiss) fed by extruder food and pellet food

      Pirali Kheyrabadi, E.; Salehi Farsani, A.; Samadi Kuchaksaraei, B. (2014)
      Food costs in rainbow trout farms are about 50% of production costs (Barrows et al., 2007). Because of inattention of most aquaculturists in exact calculation of FCR and not using qualified food, sometimes food costs will rise a lot and won't be economical. The newest method for fish food production is production during the process of extrusion. Extrusion process is processing materials with high temperature in a short time (HTST) and is done by combined operation of humidity, heat, mechanical energy and pressure (Watanabe and Pongmaneerat, 1993).
    • A preliminary study on fecundity of fresh water crayfish (Astacus leptodactylus) in Arass water reservoir, I.R. Iran

      Hosseinpour, N.; Karimpour, M. (1999)
      A research study was conducted to investigate the number of ovarian eggs and estimate potential egg production of A. leptodactylus. In this study, mean number ovarian eggs 420.41±42.51 with the average total length to 121.93±4.27 mm (n=58). The mean pleopodal eggs were 322.04±29.61 with average total length of 119.14±3.94 mm (n=67). Comparison of the fecundity rate in different population of A. leptodactylus in different areas revealed that the Arass crayfish has a greater fecundity rate than others. The discrepancy between ovarian eggs and pleopodal eggs were 21% in Arass reservoir.
    • The ability of marine Bacillus spp. isolated from fish gastrointestinal tract and culture pond sediment to inhibit growth of aquatic pathogenic bacteria

      Chen, Y.; Li, J.; Xiao, P.; Zhu, W.; Mo, Z. (2016)
      In this research, antagonistic activity of two Bacillus species isolated from digestive tract of marine fish and culture pond of sea cucumber was studied. The inhibitory activity of Bacillus spp. isolates against some common pathogenic bacteria of fish was assessed using the agar diffusion method. The strain of B. subtilis G024 exhibited antimicrobial activity against Vibrio anguillarum, V. harveyi, V. vulnificus, Streptococcus sp. and Staphylococcus aureus; the isolate of B. amyloliquefaciens N004 inhibited growth in V. anguillarum, V. campbellii, V. vulnificus, V. parahamolyticus, Edwardsiella tarda, Streptococcus sp., B. cereus. Scanning electron microscopy (SEM) investigation of indicator bacteria showed that cell morphologies were strongly affected by the cell-free supernatant of the two Bacillus spp. isolates. It is determined that the culture filtrates contained inhibitors against growth of some pathogenic bacteria with different degrees of inhibition, although none of the culture filtrates could inhibit the growth of V. fluvialis, V. alginolyticus, V. splendidus. Based upon these characteristics, both of the antagonistic Bacillus spp. isolates could be the potential probiotics used in the aquaculture production.
    • Acclimation responses of gill ionocytes of red tilapia (Oreochromis mossambicus × O. niloticus) to water salinity and alkalinity

      Jeerawat Thammaratsuntorn, J.; Jin-Liang Zhao, J.L.; Zhao, L.H.; Zhuang, Q.Q.; Guo, J.T.; Larbi Ayisi, Ch. (2016)
      To understand the acclimation strategies of red tilapia to different environments, this study aimed to evaluate different responses of red tilapia (O. mossambicus × O. niloticus) to salinity (10-30‰), alkalinity (1-3 gL-1 NaHCO3) and salinity and alkalinity (10/1-30/3 ‰/gL-1NaHCO3) environments. Localization, type, size, and numeration of gill ionocytes were investigated on the same specimens by scanning electron microscopy (SEM) and immunohistochemistry (IHC) with antibodies of Na+/K+-ATPase (NKA), Na+/K+/2Cl-contransporter (NKCC), cystic fibrosis transmembrane conductance regulator (CFTR) and carbonic anhydrase (CA). Ionocytes were only located on filaments conducted by SEM. Four types of ionocytes namely pit, convex, concave and transitory types were determined morphologically by their apical openings of which concave and transitory type were not present in freshwater (FW) and saltwater (SW) fish (10). Both ionocytes size and number increased with elevated stress levels. In comparison to FW, density of ionotypes increased to about 4.75, 3.00 and 3.44 fold in SW (30), AW (3) and S&AW (30/3) respectively. Immunoreactive cells on gill filaments confirmed branchial distribution of ionocytes. Immunoreaction of NKA, NKCC and CA appeared in FW except for CFTR while they all appeared in SW (30), AW (3) and S&AW (30/3).
    • Accumulation Variations of selected heavy metals in Barbus xanthopterus in Karoon and Dez Rivers of Khuzestan, Iran

      Mohammadi, M.; Askary Sary, A.; Khodadadi, M. (2012)
      The concentrations of Cd, Pb, Ni and Hg were determined in gill, liver and muscle of Barbus xanthopterus in down streams of Karoon and Dez Rivers during summer 2009. Heavy metal concentrations varied significantly, depending on the types of the tissue in fish species. The levels of heavy metals such as Cd, Pb, Ni and Hg in the Barbus xanthopterus of Karoon River were higher than in Barbus xanthopterus of Dez River (P<0.05). In Karoon and Dez Rivers, the concentrations of Cd, Pb and Ni were in the sequence gill>liver>muscle. The concentration of Hg in Karoon River was in the sequence liver> gill> muscle but, in Dez River, it was in the sequence liver> muscle > gill. Among heavy metals (Cd, Pb, Ni and Hg), the accumulation of Pb was more than other heavy metals in fish (P<0.05). In both rivers, the accumulation of heavy metals in muscle of fish was higher than the world health organization (WHO) standard.
    • Acute toxicity of TiO2, CuO and ZnO nanoparticles in brine shrimp, Artemia franciscana

      Khoshnood, R.; Jaafarzadeh, N.; Jamili, Sh.; Farshchi, P.; Taghavi, L. (2017)
      The brine shrimp, Artemia spp., is widely used in ecotoxicological research as a biological index. In the present study, aquatic stability and acute toxic effects of TiO2, CuO and ZnO nanoparticles (NPs) on Artemia franciscana were investigated. Acute exposure was conducted in sea water with different concentrations of selected nanoparticles at 24h, 48h, 72h and 96h. The mortality rate of A. francisca increased significantly with increasing concentrations and duration of exposure of all NPs. The toxicity pattern of metal oxides to A. francisscana was as follows: CuO>TiO2>ZnO. Our results point to the fact that both TiO2 and ZnO NPs exhibited moderate toxicity to Artemia larvae in 24h as compared with CuO, regardless of their size and concentrations.
    • Age and growth of Alburnus mossulensis (Cyprinidae) in Bibi-Sayyedan River of Isfahan Province

      Keivany, Y.; Ghorbani, M.; Paykan Heyrati, F. (2017)
      Population structure and growth of Mossul bleak, Alburnus mossulensis, a native species of cyprinids in Bibi-Sayyedan River of Semirom, Isfahan, were investigated by monthly sampling during December 2010 to December 2011. For this purpose, 543 speciments (353 females, 159 males and 31 immature) were caught using seine net (5 mm mesh size) and a cast net. Age groups of males and females were 0+ to 5+. The most frequent age group was 2+ in both sexes. The sex ratio was 1M:2.2F (p<0.01). Males outnumbered the females in younger age classes and females outnumbered the males in older age groups. The highest condition factor was observed in April for males and in March for females. The mean condition factor (CF) was not significantly different between the sexes among all fish during different months (p>0.05). The largest female was 16.80 cm in total length and 49.12 g in weight, while the largest male was 14.10 cm in total length and 28.19 g in body weight. The length-weight relationship was calculated as W = 0.0169L3.0355 (r2=0.95) for males, W=0.0061L 3.1751 (r2=0.98) for females and W=0.0066L3.139 (r2=0.99) for all fish (males, females and immature). Regression coefficient (b) based on Pauly test, was significantly different from 3 in females, but not in males (p<0.05), indicating an isometric growth pattern in males and a positive allometric growth pattern in females and all fish.
    • Age and growth of Alburnus mossulensis Heckel, 1843 in Azad Dam Reservoir and Komasi River in Kordestan Province, Iran

      Fazli, H.; Daryanabard, G.; Janbaz, A.; Naderi Jolodar, M.; Mirzaei, R.; Hoseinpour, H.; Vahedi, N. (2019)
      The aim of the present study was to estimate the population parameters including length-weight relationship (LWR), condition factor (KF), relation condition factor (Kn), age, growth and mortality of Alburnus mossulensis in Azad dam and Komasi River in Kordestan Province, Iran. Of 522 specimens, the fork length, total length and weight of A. mossulensis ranged from 70 to 164, 75-175 mm, and 4.3 to 48.1 g and averaged (±SD) 114.0 (±15.97), 127.5 (±25.29) mm and 18.2 (±5.61) g, respectively. The length-weight regression was W=0.0003×FL2.7434 indicating a negative allometric growth. The sex ratio (M:F) was 1:0.49, for adult A. mossulensis (n=134) which differed significantly from the expected 1:1 ratio (p<0.001). The von Bertalanffy growth parameters were estimated as L∞=170.3 mm, K=0.46 yr-1, t0=-0.59 yr. The instantaneous coefficient of natural mortality was estimated as 0.85 yr-1. The average of condition factor (KF) was 1.27(±0.161). Statistically significant differences were found in KF during different seasons (p<0.001). There was a significantly negative correlation between FL and KF (r2=0.60). The average of relative condition factor (Kn) was 1.06±(0.130). In the present study, the Kn of A. mossulensis were close to 1 and greater than 1 in Komasi River and reservoir, respectively. These results suggested the well-being of the fish was good in Azad Dam region.
    • Age and growth of bigeye kilka (Clupeonella grimmi Kessler, 1877) in Iranian waters of the Caspian Sea

      Janbaz, A.A.; Fazli, H.; Pourgholam, R.; Kaymaram, F.; Khedmati, K.; Parafkandeh, F.; Afraei Bandpei, M.A. (2016)
      Age and growth of Bigeye Kilka, Clupeonella grimmi, were studied in Iranian waters of the Caspian Sea from early May 2006 to April 2007. The sagitta otoliths were removed for each 5 mm length interval up to a total number of 262 fish. Fork length and weight ranged from 92.5 to 142.5 mm and 4.2 g to 23.4 g, respectively. The largest length-groups was observed from December to February and the lowest in April, which was coincided with spawning period and feeding during wintering, respectively. The overall sex ratio (male:female) was 0.33:1 which differed significantly from the expected ratio of 1:1. The length-weight regression was W= 0.0000744 FL3.14 for females and W=0.0000341FL3.16 for males, indicating the Caspian Bigeye grew isometrically for both sexes. Age determination based on otoliths readings showed that the population was composed of six- groups from 2 to 7 years old which observed a rapidly growth during the second year. In the age compositions, the four years old specimens with a mean fork length and weight 115.5±7.8 mm and 11.9 ± 2.8 g were the most abundant age group and accounted for 40.1%. The condition factor was 0.7-0.8 which varied in difference months and coincided to the gonad development. Age-at-length data were used to determine von Bertalanffy growth parameters for this population in both sexes, indicating that K value and L∞ of females was more than that of males. The results showed that C. grimmi is a rapidly-growing species in the southern parts of the Caspian Sea.
    • Age and growth of Capoeta erhani Turan, Kottelat and Ekmekçi, 2008 from the Seyhan River (Southeast of Turkey)

      Erguden, S.A.; Giannetto, D. (2016)
      Capoeta erhani is an endemic species to Southeast of Turkey. To date the information on biology of this species from inland waters of Turkey is very limited. The present study aims to provide the basic age and growth parameters of C. erhani from the middle basin of the Seyhan River. In total, 255 individuals were collected seasonally between October 2013 and September 2014. The overall sex ratio was 3.47:1 M : F. The total length of the specimens ranged from 10.4 to 33.0 cm and the weight from 9.0 to 360.0 g. The ages of total examined fish ranged from 0 to 5 years. Maximum age was found to be 5 years for both sexes. Length-weight relationship was described as logW= -5.067 + 3.168 logTL (r2= 0.972). The von Bertalanffy growth parameters were: L∞ = 32.51 cm; k = 0.400 year-1; t0 =-0.930 year for the entire population. Munro’s phi prime index in males, females and both sexes was calculated as 6.00, 6.12 and 6.04.
    • Age and growth of marbled sole Pleuronectes yokohamae in Kikonai Bay, Hokkaido, Japan

      Shafieipour, M.M.; Takahashi, T.; Ishino, K.; Takatsu, T.; Nakatani, T. (1999)
      Age and growth of marbled sole Pleuronectes yokohamae werer estimated from growth increments in otolith. The samples were collected with commercial set nets and an experimental bottom trawl net in Kikonai Bay and the nearby waters, southern coast of Hokkaido, from May 1994 to July 1995. Monthly changes in the percentage occurrence of oltoliths with translucent edge in one band and the marginal increments on the other hand verified that the translucent zone was formed once a year after their spawning season in May, indicating availability of outer margin of translucent zone as an annulus. The regression method as well as Fraser-Lee's method was used to estimate the back-calculated lengths. Growth of marbled sole was expressed by the von Bertalanffy asymptotic growth function: TLt=361.78[1-exp-0.390(t-0.098)) for males and TLt=438.15[1-exp-0.323(t-0.145)) for females using back-calculated lengths by the regression method and TLt=367.04[1-exp-0.388(t-0.114))] for males and TLt=436.05[1-exp-0.323(t-0.147)) for females by the Fraser-Lee's method, where TLt is total length (millimeters) at estimated age t.The theoretical lengths predicted by these two methods at all estimated ages were very similar. It was found that the growth rate of marbled sole in this study area was relatively high compared to those obtained in the Inland Sea of Japan as. However, the highest growth rate was observed with regard to the population off Fukushima Prefecture, although the bottom water temperatures were similar to those in Kikonai Bay. It is inferred that the geographical variation in the growth rates of marbled sole is influenced by the hydrographic and food conditions.
    • Age and growth of Sind sardine (Sardinella sindensis) using otolith from Qeshm Island (Persian Gulf)

      Dehghani, M.; Kamrani, E.; Salarpouri, A.; Kamali, E. (2015)
      This is the first study of growth and age of Sardinella sindensis from coastal waters of Qeshm Island (Persian Gulf) based on the sectioned otolith reading method. In total, 128 fishes were collected randomly from purse seine commercial nets during March 2011 to February 2012. The annual formation of the growth rings were determined by counting the opaque and translucent zones occurrence at the outer margin of the otolith. The peak of opaque and translucent zones formation occurred in June and January, respectively. There was no significant differences between the sex ratio of males to females (p>0.05). The total length and body weight for both sexes ranged between (7.9-18.6 cm) and (3.98-55.69 g). In total, four age classes were determined, and the maximum age was recorded as 3.5 years. The most abundant individuals were counted in 1+ age class, while the least number of fishes belonged to the 3+ age class. The non-linear regression relationship between the length and age for combined sexes were fitted in Von Bertalanffy growth equation, and described as: TL= 20.8 (1-e-0.54(t+ 0.54)). There was a strong relationship between the body weight and total length of the fishes (p>0.05), indicating that the growth type of S. sindensis is isometric. From the results of this study we concluded that S. sindensis is a fast-growing species with a short-life span. In order to obtain more reliable results about the age and growth of this species, we suggest that different methods of age determination should be used, combined with the evaluation of the factors affecting its growth for the management of optimum exploitation of the resources of this fish.
    • Age and growth of the Mesopotamian barb, Capoeta damascina, in central Iran

      Asadollah, S.; Soofiani, N.M.; Keivany, Y.; Hatami, R. (2017)
      Age and growth of Mesopotamian barb, Capoeta damascina, were studied in the Zayandehrud River, in central Iran, from March 2007 to April 2008. Among the 689 specimens studied, age groups in males ranged from 1+ to 9+ and that in females ranged from 1+ to 10+. The sex ratio (1M:1.6F) was not significantly different from 1:1 ratio. Maximum fork length and weight was 39 cm and 1,115 g for males and 54.2 cm and 2,340 g for females. In all age classes, females were larger than males. The most frequent age groups were 3+ in males and 4+ in females. The highest value for condition factor was observed in June. The growth of C. damascina was described by von Bertalanffy growth curve as k=0.01, L∞=56.2 cm, to=-0.63 for males and k = 0.05, L∞=117.1 cm and to =-0.43 years for females. The length-weigh relationship was described as W=0.0169L2.95 (r2=0.98) for males and as W=0.0155L2.99 (r2=0.99) for females, indicating an isometric growth pattern in both sexes. Growth performance index φ` was estimated as 5.73 for males and 6.53 for females, indicating a faster growth rate in females.
    • Age determination and feeding habits of Nemipterus japonicus (Bloch, 1791) in the northern Oman Sea

      Afshari, M.; Valinassab, T.; Seifabadi, J.; Kamaly, E. (2013)
      Age determination and feeding habits of the Japanese threadfin bream, Nemipterus japonicus, was carried out in the northern Oman Sea (Chabahar area), based on 212 specimens collected between September 2009 and May 2010. The minimum and maximum fork length and body weight were measured as 145, 258 mm and 55.31, 288.12 g. The relationship between Body Weight (BW) and Fork Length (FL) for all individuals was estimated as BW= 0.0001×FL2.83 (r2 = 0.9425, n= 212). The Vacuity Index (VI) was 55.2% that shows N. japonicus is a moderate feeder. The maximum and minimum Gastro-Somatic Index for males was in autumn and winter seasons and for females were in summer and spring. The Food Preference Indices were estimated as: crustacean (63.2%) as main food fishes (38.9%) and molluscs (36.8%) as minor food. Age determination was done by otolith sectioning. A total of 135 sagitta were sectioned. The maximum age was 5+ years old for a female with FL= 256 mm and the youngest one was 1 year old for a female with FL= 145 mm. Based on obtained results there is a significant relationship between feeding and age namely with increase of age, the feeding rate is decreased without any changes in type of feeding and food contents.
    • Age determination and morphological study using otoliths in Cyprinus carpio Linnaeus, 1758 in the Southern Caspian Sea

      Amouei, F.; Valinassab, T.; Haitov, A. (2013)
      The relationships between sagitta otolith morphology and fish size were examined for common carp in the Southern Caspian Sea and also the aging of different length groups were determined. Statistical tests to examine correlation between left and right otoliths dimensions were carried out and no statistically significant difference between them was found. The biggest otolith had 0.032g weight belongs to an individual with BW= 428g and FL=36cm and the smallest otolith had 0.014g for a fish with BW=220g and FL= 26cm. Regression model relations to each otolith morphometric parameter to fish length are provided. Also, the aging of this species was done by sectioning of sagitta otolith and a high significant correlations was found between fish age and morphometric parameters of both body weight and fork length and also otolith weight and length. The maximum age was determined 6+ years. The length-weight relationship was BW= 0.006 TL3.232 (r = 0.963). According to the morphometric relationships obtained, it was concluded that otolith length and weight are good indicators of fish body weight and fork length.
    • Age, growth and length at first maturity of Otolithes ruber in the Northwestern part of the Persian Gulf, based on age estimation using otolith

      Eskandari, Gh.; Savari, A.; Kochanian, P.; Taghavi Motlagh, A. (2012)
      Estimates of age, growth parameters, length-weight relationship and length and age at first maturity of the otolithes ruber are required for fishery management. We used counting annuli on the section of sagittal otoliths to age O.ruber from the Northwest Persian Gulf in south of Iran. Estimated ages ranged from 0 to 6 years, and maximum frequency of fishes was observed in age-group 1. The values of growth parameters L∞, k and to were calculated by von Bertalanffy model and the results were 67.57 (cm), 0.27 (year-1) and -0.43 respectively. Parameters b and an in length-weight relationship were calculated 3.19 and 0.005 respectively. Length and age at first maturity were estimated 28 cm and 1.55 year.
    • Age, growth and reproduction of Chondrostoma regium (Heckel, 1843) from the Zayandeh Roud River, Iran

      Mahboobi Soofiani, N.; Pooramini, M.; Asadollah Nasrabadi, S.; Ahmadi, S.; Hatami, R. (2014)
      Age, growth and reproduction were investigated for Chondrostoma regium, collected monthly (November 2007- August 2008) from the Dimeh Spring region, Zayandeh Roud River, Iran. The maximum age of collected female and male fish were estimated based on scale increments as five and four years respectively. Von Bertalanffy growth equations were calculated as L_t=246[1-e^(-0.206(t-0.034))], W_t=163.29〖[1-e^(-0.206(t-0.034))]〗^2.77 for females and L_t=253.1[1-e^(-0.206(t-0.175))], W_t=188.25〖[1-e^(-0.206(t-0.175))]〗^2.99 for males. Size ranges of the male and female specimens were almost similar. The fork length range for females was 10.5-18.0 cm and 10.7-19.7 cm for males and the weight range was 15.7-85.4 g in females, and 14.5-88.5 g in males. The female-male ratio was 1.8:1 (p<0.05), and for both sexes gonadal development and sexual maturity occurred in the second year of their life. Length-weight relationships revealed isometric growth pattern for males and a negative allometric growth for females. Monthly data of Gonadosomatic Index, and ovary condition suggested that spawning occurred during May and June. The mean absolute and relative fecundity was calculated as 2429±1512 (eggs/female) and 78.118.7 (egg/g), respectively, and fecundity was significantly correlated with both body weight (r2=0.90) and fork length and (r2=0.84).
    • Age, growth and spawning season of Luciobarbus esocinus Heckel, 1843 in Gamasiab River, Iran

      Hajiahmadian, M.; Rabbaniha, M.; Ghafari Farsani, H.; Gerami, M.H.; Shahbazi Naserabad, S. (2018)
      In the current paper reproductive strategy and growth rates of Luciobarbus esocinus were investigated from 2010 to 2012, in Gamasiab River, Kermanshah, Iran. Samples were collected seasonally by gillnet and electro-fisher device. Sex ratio was evaluated from 162 individuals and the percentage of male and female individuals were 54.76% and 45.24% respectively. Age structure was distributed between I and V and most frequency numbers for males and females belonged to II and IV age group, respectively. Length-weight relationship was computed as W=8E-05TL2.6546 for all individuals. Mean total length and weight was 333.82±13.10 mm and 297.67±4.85 g for males, and 371.5±21.17 mm and 307.85±7.64 g for females. The von-Bertalanffy growth models of L. esocinus were described as Lt =357.14×[1-e (-0.77× (t –0.090))] for males, Lt =367.02×[1-e (-0.78× (t –0.094))] for females and Lt =363.57×[1-e (-0.76× (t –0.087))]for both sexes. Results indicated that growth factors were highly similar in spring, summer and winter. CDA analysis revealed that the total and gonad weight are the main factors in discriminant analysis. The highest GSI values were found in spring for females and winter for males, however, it decreased rapidly after spring in females. Maximum growth rates were observed in early ages (I-II) and depleted with ages. K value and L∞ were determined as 0.77±0.09 Y-1 and 357.14±10.38 mm for males and 0.78±0.24 Y-1 and 367.02±24.15 mm for females, respectively. In conclusion this study provided basic information for stock assessment and management of L. esocinus in Gamsiab Reservoir.
    • Age, growth, sex ratio and diet of eastern mosquitofish Gambusia holbrooki Girard, 1859 in Seyhan Dam Lake (Adana/Turkey)

      Erguden, S. A. (2013)
      The aim of this study is to provide necessary information on the biology of mosquitofish in Seyhan Dam Lake in Adana, which is located in southern part of Turkey. The population structure of eastern mosquitofish Gambusia holbrooki Girard, 1859 was studied in Seyhan Dam Lake. A total of 1582 specimens (772 males and 810 females) were collected monthly from January to December, 2007. The age composition of the sample range between 0+ and 2+in both sexes. The sex ratio was M:F= 1:1.04. The length-weight relationship calculated for all individuals, with W=0.0129 x L2.927. von Bertalanffy growth parameters were for males, L∞=3.31cm, K=0.6597 year-1, to =0.9483 year for females L∞=6.62cm, K=0.2369 year-1, to =0.9259 year for all samples, L∞=5.84cm, K=0.2369 year-1, to =-1.0740 year. A total of 102 stomachs were examined and total of organisms identified from alimentary canal. The diet mainly consist of Diptera (Chironomidae (pupa) 29.40% Diptera (adult) 24.50%), Egg (mosquito 14.70% different organisms 1.96%), Crustacea (Copepoda 9.80%, Cladocera 3.92%), Coleoptera 4.90%, Hemiptera 1.96%, Fishes (G. holbrooki 3.92%), Other Hymenoptera 1.96%, Tricoptera 0.98%, Plecoptera 0.98%, Formicidae 0.98%. These data were conducted to compare with the results of other studies to carry out for other geographic areas.