Recent Submissions

  • Effect of Oxadiargyl Herbicide on Physiological, Biochemical and DNA Damage in Common carp (Cyprinus carpio)

    Somayeh, Shariatzadeh Khorasani; Hossein, Emadi; Jamili, Shahla; Mashinchiyan, Ali (Islamic Azad University, 2018)
    In this research, the effects of oxadiargyl herbicide on physiological, biochemical, and DNA destruction rates of Caspian carp, (Cyprinus carpio) were investigated. For this purpose, 550 specimens of carp were obtained (weighed 15 ± 0.8 g) duration of research was 60 days. Temperature of water was 23-27 C°. The acute toxicity test was performed over a period of 96 hours based on OECD 203 (1992) protocol, and mortality results were recorded at 0, 24, 48, 72 in 96 hours. Chronic toxicity test was performed on the basis of the OECD 204 protocol during a 30-days period. During this period، clinical symptoms and unusual behaviors of fish and important water factors were recorded. Blood sampling was performed after 10, 20, and 30 days of adaption in order to assess the cellular and biochemical parameters of blood. To prepare tissue samples (gills, liver, kidneys, muscles, spleen), after incision, separation and dehydration, clarification and paraffin emulsion were done. Paraffin blocks were prepared and with microtome,sections were prepared 6 micron thickness. Then, these sections were stained with hematoxylin-eosin (H & E). The sections of the tissue prepared with the Japan Nikon Optical Microscope, the Eclipse E600 model were examined and photographed. DNA damage was measured by alkaline comet assay. Data analysis was performed using SPSS software version 17, one-way analysis of variance and Duncan test at the level of 0.05 (mean ± SD). Based on the results, acute toxicity (LC50) was obtained at 0.6 mg/L concentration and chronic liver toxicity was obtained at 0.1, 0.3 and 0.5 mg / L. The Concentration of 0.5 mg/L treatment had the lowest levels of glucose in all of the three periodsections of experiment. Highest amounts of alanine aminotransferase, spartan transaminase and alkaline phosphatase were observed in 0.5 mg/L treatment for all of three experimental sections. The highest total protein and albumin and the lowest cholesterol in the control treatment were obtained. After examination of biochemical parameters of blood, the highest number of red blood cells was obtained in control treatment and the highest number of white blood cells was obtained in 0.5 mg /L treatment. Histological images showed that fish exposed to various concentrations of oxadiargyl herbicide obtained pathologic lesions in most tissues. The lowest levels of hematocrit and hemoglobin were obtained in 0.5 mg / L treatment. In the kidney, hemorrhage in the interstitial tissue, an increase in the number and size of melanomacrophage centers and coverage epithelium cells necrosis were observed in urinary tract tubes. In the liver, the centers of elanomacrophage increased with the myofibrils being separated, and glomeruli and corrugation were observed in most myofibrils. Based on the average DNA degradation results, the highest rate of degradation was observed in the subterminous concentration of 0.3 mg / L (20.5%).
  • Effect of Oxadiazon Herbicide on Physiological,Biochemical and DNA Damage in Common carp (Cyprinus carpio)

    Zanjani, Seyede Asal; Emadi, Hossein; Jamili, Shahla; Mashinchian, Ali (Islamic Azad University, 2018)
    This study conducted to evaluate the toxic effects of a widely used oxadiazon herbicide,on common carp (Cyprinus carpio) fingerling (15 g). The 96-h LC50 was determined, and the growth performance, serum biochemical and hematological parameters, DNA damage of erythrocytes cells, as well as histopathological changes were also determined in fish exposed to sublethal concentrations (1, 1.5 and 2 ppm) of the herbicide for 30 days. The 96-h LC50 to C. carpio was found to be 3.9 ppm. Weight gain (WG), specific growth rate (SGR) and condition factor (CF) were decreased, while food conversion ratio (FCR) increased in the exposed fish groups (P<0.05). Exposure to oxadiazon significantly increased serum ALT(Alanine transaminase), AST(Aspartate transaminase), ALP(Alkaline phosphatase), and glucose levels, while total protein, cholesterol, and albumin were significantly decreased in the exposed fish groups depend on oxadiazon concentrations and exposure time (P< 0.05). Erythrocytes cells of fish exposed to 1, 1.5 and 2 ppm of oxadiazon showed DNA damage (21.3%, 22.9%, and 28.4%, respectively) , significantly higher than control group (P< 0.05). Moreover, exposure to oxadiazon significantly decreased WBC, RBC, Hb, Hct as well as serum albumin, glucose, and total protein levels, while serum ALP was significantly increased in the exposed fish groups (𝑃�< 0.05). No significant differences were found in MCV, MCHC and MCH levels between oxadiazon treatments and control groups (P> 0.05). Oxadiazon was also induced marked histological changes included hyperemia, hyperplasia of lamellar epi-thelium, inflammatory cell infiltration, aneurysm, rod-like structures of secondary gill lamellae in the gills; fat changes in the hepatocytes, fibrosis, focal inflammatory cells and fibrocyte aggregation, diffuse and focal necrosis, hyperemia and hemorrhage and bile duct hyperplasia in the liver; hyperemia, necrosis of tubular epithelium and proteinuria in the kidney; myofibril change, necrosis, and inflammatory cell infiltration in the muscles. Changes in size and number of melanomacrophage centers were also observed in the kidney, spleen, and liver tissues of exposed fish. This study indicated that oxadiazon is highly toxic to common carp and exposure to this herbicide lead to adverse effects on the fish.
  • Histological, morphological and physiological studie on the ontogenic development of digestive tract in Alburnus chalcoides

    Zakeri-Nasab, Masrour; Jamili, Shahla; Vali-pour, Alireza; Fatemi, Seyyed Mohammadreza; Ramezani-Fard, Ehsan (Islamic Azad University, 2018)
    Alburnus chalcoides is a member of the Cyprinidae, which is considered as biologically, ecologically and economically important fish. Sampling of this fish was done randomly from the first day after hatching to pre-puberty at different intervals. The growth of this fish was investigated morphologically and histologically by Hematoxylin-Eosin staining method, and the secretion of gastrointestinal mucosal layers of the cells from carbohydrates was studied by histochemical analysis by periodic acid scavenging. To study the physiology of fish, its digestive enzymes were studied. Anatomical study on this fish showed that its digestive system consists of two major parts of the esophagus and intestines. In the early days of the digestive tract, it was a simple and simple tube in which mucosal secretions were not observed, but after the appearance of the Goblet cells, this activity began and a layer of neutral polysaccharide compounds was observed on the gastrointestinal tract. The presence of neutral polyunsaccharide secretions in the gastrointestinal mucosa has shown that it plays a role in the digestion and absorption of food and is important in protecting the epithelial layer of the digestive tract and passing food. In enzymatic studies, it was found that all enzymes had a significant difference in the level (p <0.05). The trypsin, chymotrypsin, and amino-peptidase enzymes have been shown to be more prominent in the two active start-ups, and the third and fourth weeks (entering the infant's age and adolescence). The alkaline phosphatase enzyme has a relatively stable trend and increased on the fifth day (mouth opening) and decreased slightly from week 4. Lipase and amylase also have a decrease trend, which is likely to be linked to a high protein diet of the animal. The start of active nutrition was increased, evolutionary characteristics of this fish were somewhat similar to other fish of the carp species, but there were also some differences.
  • Population dynamic and biological characteristics of Longnose trevally Carangoides chrysophorys in the Persian Gulf & Oman Sea (Hormozgan Province)

    Hashemipour, Fariborz; Keymaram, F.; Kamrani, E.; Jamili, Shahla; Ramezanifard, E. (Islamic Azad University, 2079-10)
    The long nose trevally, Carangoides chrysophrys is one of important commercial species of family carangidae in the Persian Gulf and Oman Sea .population dynamic parameters, reproductive biology and feeding habits of this species were studied from April 2016 to march 2017 in the Coastal Waters of Hormuzgan province. Length frequency analysis of 440 specimens of C.chrysophrys with total length (TL) ranged from 250 to 800 mm were shown isometric growth. Growth parameters including K, L and t0 were calculated 0.390 (1/year), 66.86 Cm and 0.029 Year respectively. The instantaneous rate of total mortality (Z), natural mortality (M) and fishing mortality (F) were estimated 1.21, 0.761 and 0.449 (1/year) respectively. Exploitation rate was 0.4 per year indicated that fishing effort on this species is in a moderate condition. Reproductive studies showed that C.chrysophrys has long spawning period with two peaks in spring and autumn respectively. Females reached 50% sexual maturity at 38.4 cm. Stomach content analysis of this species revealed that bony fishes (% 77) formed main food and followed by crustacean (% 18) and molluscs (% 5) as random food.
  • Evaluation of the effect of chitosan and collagen extracted from aquatic(shrimp, Rutilus kutum and Scomberomorus guttatus) on wound healing

    Naderi Gharahgheshlagh, Soheila; Jamili, Shahla; Fatemi, MJ.; Sharifi, AM.; Nourani, MR. (Islamic Azad University, Science and Research Branch, 2017-09)
    his study has been conducted to extract collagen from the skin of Rutilus kutum from of the Caspian Sea and Scomberomorus guttatus which is one of the tuna species of the Persian Gulf. Acid-soluble collagens (ASC) from the skin of Scomberomorus guttatus and Rutilus kutum were extracted and characterized by Pico.Tag, SDS-PAGE, FTIR and UV spectrophotometry. Then The collagenchitosan gel extracted from aquatic (shrimp, Rutilus kutum and Scomberomorus guttatus fish) was used to evaluation II grade burn wound treatment. Burn treatment was performed in two steps: 1. First step; Determine the most effective collagen-chitosan gel ratio (collagen / chitosan ratio) with three different ratios (1: 3, 1: 1, 3: 1 Rutilus kutum fish collagen-chitosan( RKC0.75, RKC0.5 and RKC0.25) and Scomberomorus guttatus fish (SGC0.75, SGC0.5 and SGC0.25). The second step was to compare the purpose group (collagen-chitosan gel) with the control and control groups in this The rats were divided into 5 groups. The first group (N = 8): 1% collagen gel, the second group : 1% collagen-chitosan gel, the third group: 1% chitosan gel, 4th group : silver sulfadiazine, Group 5: withoud drug. Burn wound healing was evaluated by two methods of measuring wound area and histopathology. Based on the data from the SDS-PAGE and the composition of amino acid, both collagens were classified as type of collagen I. Collagens consisted of two different types of α chains, including α1 and α2, and β component. Also, FTIR analysis revealed the presence of helical arrangements of collagens. Moreover, UV spectrophotometry exhibited that this collagen had an absorbance at 220 nm. According to the basis of dry weight, the extract of collagen in Rutilus kutum and Scomberomorus guttatus ASC were calculated 15.6 % and 14.5 %. Amino acid analysis revealed a much higher glaycin content in the both collagens extracted which accounted to one third of the total amino acids. Besides, proline as an unique amino acid in Rutilus kutum and Scomberomorus guttatus ASC had the specific amount of 89.6 and 86.8 residues per 706.1 residues respectively. The results of the study to determine the most effective ratio of collagen-chitosan in Rutilus kutum based on wound area measurement showed that the group treated with collagen-chitosan gel with a ratio of 1: 1 (RKC0.5) had the greatest effect on burn wound healing and the group Treatment with RKC0.25 had less effect on burn wound healing than the other two groups. The results of the investigation of determining the most effective collagenchitosan ratio in Scomberomorus guttatus fish based on wound area measurement showed that the treated group with collagen-chitosan gel with a ratio of 1: 3 (SGC0.75) on days 5, 10, 15 and 20 And 25 burns had the least wound area than the other two groups, so it had the greatest effect on burn wound healing and the treatment group with SGC0.25 had less effect on burn wound healing than the other two groups, also the Histopathologic results showed that there was no a significant difference between the groups (SGC0.75 and SGC0.5 and SGC0.25) on 15 and 30 days. (P> 0.05). The results of the comparison purpose group (collagen-chitosan gel) with the control group and the control group in both groups Rutilus kutum and Scomberomorus guttatus fish showed that on the 10th, 15th, 20th and 25th day of burn, the treated group with collagen- Chitosan had a significant difference with the sulfadiazine treated group and the non-drug group (p <0.005). The results also showed that the treated group with collagen-chitosan gel was completely improved on day 25 and the treated group with silver sulfadiazine had the highest wound area among the groups. The results of histopathologic study showed that there was a significant difference between the groups in the 15th and 30th burns (p <0.05), and the treated groups with collagen gel, chitosan gel and collagen-chitosan gel had higher scores and better results than The two groups of silver sulfadiazine and the group did not burn at day 15 and 30 without using the drug. collagen-chitosan gel can be used as a good candid for second burn healing skin.
  • Effect of sub-accute exposure of Cu nanoparticles on histopatological, DNA damage and oxidative enzymes changes in ( Rutilus caspicus Yakovlev, 1870 )

    Aghamirkarimi, Shahrzad; Mashinchian Moradi, Ali; Sharifpour, Issa; Jamili, Shahla; Ghavam Mostafavi, Pargol (Islamic Azad University, Science and Research Branch, 2017-09)
    The current study has determined the toxicity effects of copper nanoparticles on the some vital organs such as gill, liver and kidney of Caspian Roach; Rutillus caspicus. For this purpose, 120 fishes were used as experimental fishes and exposed to 0.1, 0.2 and 0.5 mg/L of Cu nanoparticles for 21 days, and 30 fishes as control. The mean water temperature of the aquaria was 22±2 ºC, dissolved oxygen 5.2 mg/L, pH at 7±0.004 and the concentration of calcium carbonate was 270 ppm. On the 7, 14 and 21 days after exposing the fishes to Cu nanoparticles, three fish were randomly selected from each aquaria, sacrificed and samples from their gill, liver and kidney were taken and fixed in cold 10 % buffered formalin. Then microscopic sections were prepared and examined by light microscope which showed histological alternations in the gill, liver and kidney tissues. Then blood samples collected for enzymatic and DNA damage evaluation. Histological al-ternation in gills included: hyperplasia, fusion and detachment of secondary lamellae, blood congestion in vascular axis of primary filaments, reduced secondary lamellae length and cellular degeneration. Histolog-ical changes in liver included blood congestion in the central veins, cytoplasmic vacuolation of the hepato-cytes, cellular degeneration, congestion in the blood sinusoids and necrosis of the hepatocytes. Histological changes in kidneys included glomerular shrinkage, severe degeneration in the tubules cells, interstitial tissue and glomerulus, increase in interstitial tissue cells and macrophages aggregation.The most severe alternation of the gill, liver and kidney were in third weeks at 0.5 mg/l concentration. There were positive correlation between concentration and hyperplasia (r=.815, p<.01), fusion (r=.620, p<.05), detachment (r=.849, p<.01), blood congestion (r=.706, p<.05), degeneration (r=.742, p<.01). There were positive correlation between time and telangiectasia (r=.611, p<.05). There were positive correlation between concentration and hepato-cytes hypertrophy (r=.652, p< .01), necrosis (r=.768, p<.05). The activity of antioxidant stress enzymes, superoxide dismutase (SOD) and catalase (CAT), were measured in the specimens. The maximum level of enzymes activities were in first week at 0.1 mg/l concentration. There were significant difference between treatments and control (p<0.05). We used comet assay for evaluation of DNA damage. The activity of anti-oxidant stress enzymes were higher in low concentration. DNA damage was significant in all treatments. The most severe DNA damage have seen in third week at 0.5 mg/l concentration. Evaluation of these changes could be useful in estimating the harmful effects of Copper nanoparticles. The degree of damages was more intensive at higher CuNP concentrations. The result of the study showed that CuNPs could cause severe damages on the Caspian roach; Rutillus caspicus and have lethal effects for fish.
  • Comparison antibacterial activity gelatin nanoparticles and gelatin yellowfin tuna (Thunnus albacares)and determination minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC)

    Sadeghi, H.; Jamili, Sh.; Rezayat, M.; Attar, H.; Kaymaram, F. (2018)
    With the now diseases and increasing in the antibiotics has been greatly improved to deal with the increasing drug resistance of pathogens. Nanotechnology is a useful way. The aim of this article is to evaluate the gelatin antibacterial property and gelatin nanoparticles against pathogenic bacteria such as E. coli, S. aureus, and P. aueroginosa and were evaluated by calculation of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Gelatin and gelatin nanoparticles were extracted from yellowfin tuna. For antibacterial assay gelatin was disc diffusion and serial dilution methods. For gelatin nanoparticles antibacterial assay was turbidimetric method. Then evaluated antibacterial characters and MIC MBC were determinated. (Comparison gelatin with gelatin nanoparticles antibacterial characters showed that gelatin nanoparticles had antibacterial characteristics against S. aureus, and P. aueroginosa and gelatin had property against P.aueroginosa. Gelatin nanoparticles had lower than MIC and MBC gelatin (P=0/0 24).Gelatin nanoparticles had better antibacterial property than gelatin. The smaller the Gelatin nanoparticles (GNP), the more antibacterial characters (P< 05/0). GNP could be used pharmaceuticals and have antibacterial properties.
  • The study of antibacterial nanoparticles obtained from yellowfin tuna (Thunnus albacares)

    Sadeghi, H.; Jamili, Sh.; Rezayat, M.; Attar, H.; Kaymaram, F. (2016)
    One of the mainly valuable consumed colloid protein materials in pharmaceutics, medical and food industries is Gelatin. Fish gelatin near warm water is similar mammal gelatin. Due to the amount of catch of yellowfin tuna (Thunnus albacares) use in factory. Fish skin are peel off and wasted in factory every day. Analysis factors were extracted alkaline method gelatin from skin, physiochemical and rheological test (amino acid composition (HPLC), electrophoreses, fourier transform infrared, moisture content, pH, setting point , setting time, melting point and melting time, color and gelatin yield) with access method of National Iran Standard . Prepared gelatin nanoparticles from gelatin with desolvation method. Determination of particles size, size distribution, zeta potential for characterize the surface and morphology .Antibacterial assay with disc diffusion method and determined MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) for Esherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. For nanoparticles gelatin antibacterial assay (MIC and MBC) turbidimetry. Statistical processing were linear regression and modeling and forecasting regression. Albeit antibacterial compared gelatin and gelatin nanoparticles.It produced 0.034g gelatin for one gram of yellowfin tuna. Yellowfin tuna had higher gelatin content (Proline and Hydroxyproline) than mammalian gelatin content. SDS-electrophoresis for yellow fin gelatin showed protein band (α, β, γ) same as mammalian protein band. Fourier transform infrared had the same spectra for both of them. Factors were pH (6.1), Moisture (8.5%) Setting temperature and time respectively 4(c) and 60(s) and Melting temperature and time respectively were 50(c) and 45(s). The color was transparent. The mean size of the gelatin nanoparticles was 132 nm and PI (polydispersity) and zeta potential were respectively 0.248, -31 MV. pH, speed of addition of acetone, percent of glutaraldehyde depends on size of nanoparticles gelatin. (Statistically significant was P<0.05). Gelatin nanoparticles had antibacterial in Pseudomonas aeruginosa and Staphylococcus aureus while gelatin had antibacterial Pseudomonas aeruginosa. Nanoparticle gelatin had higher MIC and MBC than gelatin. Statistically significant was P<0.05. Antibacterial assay for gelatin particles has evaluated for foodstuffs packing and it may using drug delivery. This kind of gelatin is lawand similar mammalian gelatin. Antibacterial properties is more useful for usage in foodstuffs packing and may using drug delivery.
  • Acanthobrama microlepis De Filippi, 1863

    Jamili, Sh.; Ketabi, R. (Islamic Azad UniversityTehran, Iran, 2019-01)
    Location in Iran: River Basin and Caspian Sea Basin. References: Teimori, A., Esmaeili, H.R., Sayyadzadeh, G., Zarei, N., Gholamhosseini, A. 2015. Molecular systematics and distribution review of the endemic cyprinid species, Persian chub, Acanthobrama persidis (Coad, 1981) in Southern Iran (Teleostei: Cyprinidae). Molecular Biology Research Communications, 4(4):189-206. Jouladeh-Roudbar A., Vatandoust S., Eagderi S., Jafari-Kenari S., Mousavi-Sabet H., 2015 Freshwater fishes of Iran; an updated checklist. AACL Bioflux 8(6): 855-909. Ramin M., Valinassab T., Dostdar M., Jamili Sh., 2018. Management of genetic resources vertebrates in the Inland waters of Iran. Iranian Fisheries Science Research Institute. 45pp.
  • Acanthobrama marmid, Heckel, 1843

    Jamili, Sh.; Ketabi, R. (Islamic Azad UniversityTehran, Iran, 2019-01)
    Location in Iran: Kor, Mond, and Kul rivers, Tigris River basin, The border of Iraqi marshes in Iran. References: Coad, Brian W. 2015. Review of the freshwater catfishes of Iran (Order Siluriformes). Iranian Journal of Ichthyology, 1(4) (2014): 218-257. Jouladeh-Roudbar A., Vatandoust S., Eagderi S., Jafari-Kenari S., Mousavi-Sabet H., 2015 Freshwater fishes of Iran; an updated checklist. AACL Bioflux 8(6): 855-909. Ramin M., Valinassab T., Dostdar M., Jamili Sh., 2018. Management of genetic resources vertebrates in the Inland waters of Iran. Iranian Fisheries Science Research Institute. 45pp.
  • Abramis brama Linnaeus, 1758

    Jamili, Sh.; Ketabi, R. (Islamic Azad UniversityTehran, Iran, 2019-01)
    Location in Iran: Caspian Sea; Urmia Lake basins; Anzali Wetland; Sefidroud River. References: Coad, Brian W. 2015. Review of the freshwater catfishes of Iran (Order Siluriformes). Iranian Journal of Ichthyology, 1(4) (2014): 218-257. Jouladeh-Roudbar A., Vatandoust S., Eagderi S., Jafari-Kenari S., Mousavi-Sabet H., 2015 Freshwater fishes of Iran; an updated checklist. AACL Bioflux 8(6): 855-909. Ramin M., Valinassab T., Dostdar M., Jamili Sh., 2018. Management of genetic resources vertebrates in the Inland waters of Iran. Iranian Fisheries Science Research Institute. 45pp.
  • Acanthalburnus urmianus Günther, 1899

    Jamili, Sh.; Ketabi, R. (Islamic Azad UniversityIslamic Azad University, Science and Research Branch,Tehran, 2018-12)
  • Silurus Linnaeus

    Ketabi, R.; Jamili, Sh. (2018)