Recent Submissions

  • Feeding habits of the scalloped spiny lobster, Panulirus homarus (Linnaeus, 1758) (Decapoda: Palinuridae) from the South East Coast of Iran

    Mashaii, Nassrin; Rajabipour, Farhad; Shakouri, Arash (2011)
    Certain ecological aspects of the feeding habits of 260 scalloped lobsters Panulirus homarus collected during monthly dives off the southeast coast of Iran were considered. Parameters under consideration included: water temperature, salinity, turbidity, pH, dissolved oxygen and macrobenthose of the lobsters habitat. The mean number of empty stomachs varied from 25% to 62%. Statistical analysis revealed no monthly or seasonal significant difference in the frequency of non-empty stomachs (P>0.05). 1 to 6 prey items were distinguishable in each non-empty stomach. Monthly mean of point counts of prey obtained was from 8.76±4.18 to 154.95±73.62, without any significant difference by Tukeys’ HSD test. Bivalves often had the maximum amounts of Fi and Pi amongst different prey items. Pearson χ2 test did not show any significant difference between frequency of occurrence of each prey item with different length classes, sex, moulting condition and presence of eggs in females (P>0.05). Ivlev electivity index of crabs, gastropods and bivalves was close to 1 and sloped to -1 for polychaetes and echinoderms. This index revealed crabs, bivalves and gastropods were more important in the diet than sediment throughout the year. Frequencies of food occurrence suggest bivalves as the main food; crabs, gastropods, barnacles and algae as secondary food and polychaetes, fish, echinoderms and Ascidiacea as incidental food for P. homarus in the area. Spearmans’ correlation coefficients of stomach fullness against different length classes, sexes, moulting and ovigerous females were not significantly different (P>0.05). There was only a significant Pearsons' correlation between the number of point for bivalves in the stomach against body weight (P<0.05).
  • Effect of pituitary extract, ovaprim and combination of human chorionic gonadotropin and metoclopramide on reproductive performance of Caspian shemaya, Alburnus chalcoides (Guldenstadt, 1772)

    Nosrati, M.; Khara, H.; Vahabzadeh, H.; Mirhashemi Nasab, S.F. (2019)
    The present study was conducted to examine the effects of three different hormonal treatment types i.e., pituitary extract (Pt), ovaprim (Ov) and human chorionic gonadotropin (HCG) + Metoclopramide (Met) on reproductive performance of Caspian shemaya, Alburnus chalcoides (Guldenstadt, 1772). To this end, three experimental treatments and one control group were considered. The experimental groups were administrated with different doses of Pt, Ov, HCG + Met as follows: Pt [2 mg kg bw (body weight)^-1], Pt (3 mg kg bw^ -1), Pt (4 mg kg bw^ -1), Ov (10 μg kg bw^ -1), Ov (20 μg kg bw^ -1), Ov (30 μg kg bw^ -1), HCG + Met (1000 IU kg bw^ -1), HCG + Met (2000 IU kg bw^ -1), HCG + Met (3000 IU kg bw^-1) and also a control group without any hormonal treatment. The highest values of oocyte weight and egg diameter were observed in groups administrated with Ov (10 μg kg bw^-1) and HCG + Met (2000 IU kg bw^-1) respectively. The highest values of absolute fecundity and relative fecundity were recorded for fish administrated with Ov (20 μg kg bw^-1). The latency period and hour-degree for final maturation were lower in fish administrated with Ov (10 μg kg bw^-1) compared to other experimental groups (p<0.05). There were no significant differences between experimental groups in terms of other assayed parameters. The results of the present study demonstrated higher efficiency of ovaprim in improving the reproductive performance of Caspian shemaya
  • Fishery and population dynamics of Caspian kutum, Rutilus frisii kutum (Cyprinidae), in the Caspian Sea

    Afraei Bandpei, Mohammadali (Universiti Sains Malaysia, 2010)
    The Caspian Sea is the largest enclosed body of water in the world. The water is slightly brackish. The aim of project was to determine length at maturity (Lm_50%), evaluate of feeding items, investigation some of biological aspects included in sex ratio, growth and age structure, instantaneous mortality coefficient, maximum sustainable yield (MSY) and break-even point of Rutilus frisii kutum in southern part of the Caspian Sea. Specimens of Kutum were collected from the catches obtained from 131 sample sites along the Iranian coast of the Caspian Sea. The minimum and maximum fork length and weight was 21cm and 58cm with mean 38.4±6.4 cm; 104 and 2450 g with mean 775.2±382.5 g, respectively. The sex ratio of males to females was 0.65: 1 (males: females). The condition factor in Guilan was more than Mazandaran and Golestan provinces. The length at maturity (Lm_50%) of Kutum obtained was 39.1cm. The minimum and maximum absolute fecundity varied from 15,713 to 130,737 eggs with mean 69,961.7±3,836.4. The reproduction phase was extended from February to April, peak in March, with the highest average values 5.52 for males and the highest value was 17.00 for the females in April. Due to males‟ migration from sea to river occurred sooner than that of the females. The diet consisted of different prey items, bivalves representing the most important prey group (59%), and followed by Cerastoderma lamarki (57%). The instantaneous natural mortality rate was estimated M= 0.46 y^-1. Based on total instantaneous mortality coefficient (Z) from the seasonalized length-converted catch xxv curve, F (the instantaneous fishing mortality coefficient) as 0.82 y^-1 through the relationship: F=Z-M, and giving a current exploitation rate E (=F/Z) as 0.64 y^-1. The length-at-first capture and age-at-first capture was obtained L_c =36.8 cm and 3.92 years, respectively. The Maximum sustainable yield (MSY) based on cohort analysis for Kutum resulted MSY=20,004 tonnes with biomass B of 51,937 tonnes. The selection ogive procedure gave the following summary: E_max=0.76, E_0.1=0.65 and E_0.5=0.39. Yield per recruit analysis suggests that the stock of this species has a moderately exploitated at E=0.64. Kutum contributed about 76% of total sell bony fish in south of the Caspian Sea and total income of Kutum sell were estimated US$30,415,998. The income of the fishermen through selling of Rutilus frisii kutum from total bony fish income was 74% for Guilan, 85.8% for Mazandaran, and 26% for Golestan provinces. Therefore, R. f. kutum plays a significant role in fishermen‟s income in southern coasts of the Caspian Sea. The break-even point of Kutum obtained with mean 19,220±5,364.5 for Guilan, 18,843±4,989.2 for Mazandaran, and 10,677±1,358.2 for Golestan provinces, which coincided with the fishing effort, catch ratio, number of fishing cooperatives, and number of labour.
  • Evaluation of toxicity and biochemical effects of the Oxadiargyl in Common carp (Cyprinus carpio L.)

    Emadi, Hossein; Shariatzadeh, Somayeh; Jamili, Sh.; Mashinchian, Ali (2018)
    This study aimed to investigate the effects of sublethal concentrations of oxadiargyl herbicide on growth performance, serum biochemical parameters and liver histology of the common carp, Cyprinus carpio, during 30 days exposure period. Carp fingerlings were randomly divided into four duplicate groups. Experimental groups were exposed to the concentration of 0.1, 0.3 and 0.5 ppm of oxadiargyl, while the control group was kept in toxicant free. During the experiment, none of the control fish died. Weight gain and condition factor decreased, while food conversion ratio increased in fish exposed to 0.5 ppm of oxadiargyl. Exposure to oxadiargyl significantly increased serum ALT, AST and ALP, as well as glucose levels, while cholesterol, total protein and albumin were significantly decreased in the exposed fish groups depend on oxadiargyl concentrations and exposure time. Diffuse and focal necrosis mainly as perivascular necrosis fibrosis, increase in size and number of melanomacrophage centers, bile duct hyperplasia, hyperemia and hemorrhage, fatty changes in the hepatocytes, fibrocyte aggregation and focal inflammatory cells were also the histological lesions observed in the liver of exposed fish. These results showed that oxadiargyl is highly toxic to common carp and had negative effects on the growth performance, serum biochemical parameters and the liver tissue of common carp.
  • The 12th Meeting Aquaculture Departments of IFRO and it's dependent Centers

    Sahhafi, H.H.; Jamili, Sh.; Matinfar, A.; Mazloomi, M.; Moazedi, J.; Gharra, K.; Hosseini, M.R.; Hasanniya, M.R.; Abdolhai, H. (Iranian Fisheries Science Research InstituteTehran, Iran, 2007)
    Without abstract.
  • Effect of Diazinon on Catalase Antioxidant Enzyme Activity in Liver Tissue of Rutilus rutilus

    Keramati, Vida; Jamili, Sh.; Ramin, M. (2010)
    Diazinon on as an organophosphate toxin applied widely in agricultural gardens of Iran, so every year huge volume of diazinon on enter to aquatic ecosystems. In this study, antioxidant enzyme activity (Catalase) were assessed in liver of Rutilus rutilus weighing about 7.0+1.0 g following exposure of fish to 20, 40 and 60 lig L-1 concentration of diazinon at 22.0+1.0°C and acceptable water quality conditions. At the first, LC„ 96 h was determined 4.5 ppm. We have 3 treatments with 3 replicate and 1 controls (without diazinon). on). In each aquarium (Volume: 8 L) were 10 fishes. The result of measuring catalase activity in liver with special kit did not show significant differences between treatment groups and control (p>0.05). Also, regression curve did not show significant correlation between increasing toxin concentration and catalase activity changes. The results showed that in same concentration, catalase activity in time 24 h significantly was higher than times 48 and 96 h (p<0.05). Finally, the results did not show special relationship between different concentrations of diazinon on and catalase activity in fish liver, that is maybe was result of parameters such as antioxidant enzymes conjugative operation, enzymes professional reaction against special pollutant, rapid reactions and acute phase reaction and antioxidant adaptation again. st pollution and antioxidative reaction outbreak in target organ.
  • A note on the cinnabar goatfish, Parupeneus heptacanthus (Lacepède, 1802) from northern parts of the Persian Gulf and the Makran Sea (Teleostei: Mullidae)

    Ketabi, Ramin; Esmaeili, Hamid Reza; Echreshavi, Sorour; Jamili, Shahla (2017)
    The cinnabar goatfish, Parupeneus heptacanthus (Lacepède, 1802), is a species of mullid fish distributed in the Red Sea, Indo-West Pacific: East Africa, Madagascar and Mascarenes east to Marshall Islands, Samoa and Tonga, north to southern Japan, south to Australia, Lord Howe Island and New Caledonia. It has been mainly reported from the southern part of the Persian Gulf and Makran Sea. Here, the morphological features especially live color pattern of P. heptacanthus from the northern area of Persian Gulf and Makran Sea are described and discussed. It is the first record of life color pattern of this species from the northern Persian Gulf.
  • Morphology and phylogeny of the sea anemone Stichodactyla haddoni (Cnidaria: Anthozoa: Actiniaria) from Chabahar Bay, Iran

    Attaran-Farimin, Gilan; Javid, Pegah; Shakouri, Arash (2015)
    Carpet anemones of the genus Stichodactyla are characterized by having no calcium carbonate skeleton, being flattened with rather short tentacles, being exclusively tropical, and being hosts for clown fish species. Most of the species belonging to this genus, however, are often very similar morphologically; thus, identification by external features is rather difficult. In this study, we have described the morphology and phylogenetic affinities of a carpet anemone species collected from Chabahar Bay. Although the Iranian species showed different coloration patterns within the same geographical area, they strongly resemble Stichodactyla haddoni Saville- Kent, 1893 in having short tentacles densely covering the undulated oral disc and white, pointed, recognizable exocoelic tentacles. Comparison of 18S rDNA with other actiniarians revealed that the 2 Iranian species are closely related to Stichodactyla haddoni, Stichodactyla gigantea, and Heteractis magnifica. This is the first record of Stichodactyla haddoni from the southeastern coast of Iran and the northern part of the Sea of Oman.
  • Antimicrobial Activity of Sea Cucumber (Stichopus variegatus) Body Wall Extract in Chabahar Bay, Oman Sea

    Shakouri, Arash; Shoushizadeh, Mohammad Reza; Nematpour, Fatemeh (2017)
    Seas and oceans have been recognized as a rich source of metabolites and bioactive compounds with biodiversity and drug activity. These compounds have accumulated in various organisms. Bioactive compounds can be extracted from various animal groups, such as coral, crabs, moss animals, Echinodermata, case-bearers, fishes, and sponges. Objectives: Antimicrobial extracts of the sea cucumber body wall (Stichopus variegatus), collected from Chabahar Bay, were tested for four bacterial, fungal, and yeast species. Methods: The disk diffusion method was used in this study. The McFarland 0.5 standard was used in the preparation of suspensions of the mentioned microorganisms under a hood. Next, the species were cultured with a swab on a Mueller-Hinton agar for bacteria and on a Sabouraud dextrose agar for fungi. Subsequently, the disks, with appropriate concentrations, were placed on microbiological growth media. Results: The results of the study showed that the best effect belonged to the aqueous methanol extract of the sea cucumber body wall, with an 8 mg/mL concentration on E. coli, with an inhibition zone diameter of 12.26 mm. The lowest inhibition zone diameter belonged to the methanol extract of white strands, with a 2 mg/mL concentration on C. albicans and an inhibition zone diameter of 1.16 mm. The n-hexane and chloroform extracts had no effect. Conclusions: Comparison of these tests indicates that sea cucumbers have an innate immunity system, which can be considered a potential source for discovering antimicrobial peptides.
  • New Observation of Three Species of Sea Cucumbers from Chabahar Bay (Southeast Coasts of Iran)

    Shakouri, A; Aminrad, T; Nabavi, M.B; Kochanian, P; Savari, A; Safahiye, A (2009)
    Three species of sea cucumber (2 species belong to genus Holothuria and one species of Stichopus) were collected on subtidal zone of Chabahar Bay in the late of 2007. The literature review on the distribution was revealed that this is the first report of H. leucospilota, H. arenicula and S.variegatus from Chabahar Bay (Sea of Oman). The species identification was done through morphological keys and review of their ossicles. This study is revealed the special characteristics of the presented species in order to just identification of them
  • New Observation of Two Species of Sea Cucumbers from Chabahar Bay (Southeast Coasts of Iran)

    Shakouri, A; Nabavi, M.B; Kochanian, P; Savari, A; Safahieh, A; Aminrad, T (2009)
    Although, sea cucumbers are well known animals in Eastern Asia, in Iran they are not popular marine animals. Divers recorded these animals in their dives but this is the first scientific approach in identification of holoturians in Southeast coast of Iran. All sea cucumbers were collected with SCUBA diving and species identification was done through morphological keys and review of their dermal ossicles. There are two species of sea cucumber belong to genus Holothuria were collected on subtidal zone of Chabahar Bay in the late of 2007. This is the first report of H. hilla, H. parva from Chabahar Bay (North of Oman Sea). This study is revealed the special characteristics of the presented species in order to just identification of them. In the studied areas, H. parva has known as a rare species.
  • The First Report of Micronephthys Sphaerocirrata: Nephtyidae in Sub-Tidal Areas of Chabahar Bay

    Shakouri, Arash; Dehani, Esmaeil (2015)
    This study was conducted to identify Micronephthys sphaerocirrata species in sub-tidal areas of Chabahar Bay during the time period from June 2013 to February 2014. The study area is located in south- east of Iran Oman - Sistan and Baluchistan coast. The sampling was performed from Shahid Beheshti, Hafte Tir and Konarak ports. The samples were taken seasonally from each port three times from the depths of 2- 10 meters by Van-Veen grab (250 cm2) for identification and one liter of sediment was taken to determine the grain size of the sediment habitat M. sphaerocirrata. The samples were sent to the laboratory and the identifications were performed based on the necessary resources and references. Also the environmental factors such as pH, Salinity, temperature in the sample were determined. The results of this study indicated that M. sphaerocirrata species had the highest frequency during autumn.
  • Sea star (Echinodermata) species diversity and distribution in the south-east of Iran (Gulf of Oman)

    Pourvali, Naser; Esfandiar pour, Fatemeh; Shakouri, Arash; Rezai, Hamid (2014)
    Echinoderms are one of the oldest and most important groups of marine organisms which play an important role in the marine ecology especially in the coral reefs. During November 2012 to August 2013, six stations were selected in intertidal zone at the north of Gulf of Oman according to sea bottom. At each station, the Sea star species of the selected area were counted in 10 m2 with 10 replications. This study was made in three seasons namely autumn, winter and summer. According to the result, cluster analyses showed the site similarity in species abundance and diversity with the same substrata. Maximum and minimum sea star diversity was shown in winter and summer, respectively.
  • Antibacterial and Antifungal Activity of Holothuria leucospilota Isolated From Persian Gulf and Oman Sea

    Adibpour, Neda; Nasr, Farhad; Nematpour, Fatemeh; Shakouri, Arash; Ameri, Abdolghani (2014)
    Emergence of antimicrobial resistance toward a number of conventional antibiotics has triggered the search for antimicrobial agents from a variety of sources including the marine environment. Objectives: The aim of this study was to evaluate the antimicrobial potential of Holothuria leucospilota from Qeshm and Kharg Islands against some selected bacteria and fungi. Materials and Methods: In this investigation, sea cucumbers from two coastal cities of Persian Gulf were collected in March and May 2011 and identified by the scale method according to the food and agriculture organization of the United Nations. Antibacterial activity of hydroalcoholic extracts of the body wall, cuvierian organs and coelomic fluid, methanol, chloroform, and n-hexane extracts of the body wall were evaluated by the spot test. In addition, their antifungal activity was assessed by the broth dilution method. Results: The displayed effect was microbiostatic at concentrations of 1000 and 2000 µg/mL rather than microbicidal. The highest activity of hydroalcoholic extracts was exhibited by body wall, cuvierian organs and coelomic fluid against Escherichia coli, Salmonella typhi, Staphylococcus aureus and Pseudomonas aeruginosa; Aspergillus niger, A. fumigatus, A. flavus and A. brasilensis. However, none of the methanol, chloroform and n-haxane extracts showed appreciable effects against Shigella dysenteriae, Proteus vulgaris, Bacillus cereus, S. epidermidis and Candida albicans. Moreover, cuvierian organs did not possess any antifungal potential. Conclusions: Our data indicated that water-methanol extracts from the body wall of H. leucospilota possess antibacterial and antifungal activity. However, additional and in-depth studies are required to isolate and identify the active component(s)
  • Sublethal effects of copper nanoparticles on the histology of gill, liver and kidney of the Caspian roach, Rutilus rutilus caspicus.

    Aghamirkarimi, Sh.; Mashinchian Moradi, A.; Sharifpour, I.; Jamili, Sh.; Ghavam Mostafavi, P. (2017)
    The current study has determined the toxicity effects of copper nanoparticles on the some vital organs such as gill, liver and kidney of Caspian Roach; Rutillus rutillus caspicus. For this purpose, 120 fishes were used as experimental samples and exposed to 0.1, 0.2 and 0.5 mg/L of Cu nanoparticles for 21 days, and 30 fishes assumed as the experiment control. The mean water temperature of the aquaria was 22±2 ºC, dissolved oxygen 5.2 mg/L, pH at 7±0.004 and the concentration of calcium carbonate was 270 ppm. On 7, 14 and 21 days after exposing the fishes to copper nanoparticles, three fishes were randomly selected from each aquaria, sacrificed and samples from their gill, liver and kidney were taken and fixed in cold 10 % buffered formalin. Then microscopic sections were prepared and examined by light microscope which showed histological alternations in the gill, liver and kidney tissues. Evaluation of these changes could be useful in estimating the harmful effects of copper nanoparticles. Histological alternation in gills included: hyperplasia, fusion and detachment of secondary lamellae, blood congestion in vascular axis of primary filaments, reduced secondary lamellae length and cellular degeneration. Histological changes in liver included blood congestion in the central veins, cytoplasmic vacuolation of the hepatocytes, cellular degeneration and congestion in the blood sinusoids and necrosis of the hepatocytes. Histological changes in kidneys included glomerular shrinkage, severe degeneration in the tubules cells, interstitial tissue and glomerulus, increase in interstitial tissue cells and macrophages aggregation. The degree of damages was more intensive at higher copper nanoparticles concentrations. The result of the study showed that copper nanoparticles could cause severe damages in the vital tissues of Caspian roach; Rutillus rutillus caspicus and have lethal effects for fish.
  • Effect of TiO2 nanoparticles on the physico-mechanical and ultraviolet light barrier properties of fish gelatin/agar bilayer film.

    Vejdan, Akbar; Ojagh, Seyed Mahdi; Adeli, Afshin; Abdollahi, Mehdi (2016)
    Bilayer gelatin/agar films containing different concentrations of TiO2 (0.5, 1, and 2 g/100 g) were prepared by incorporation of anatase titanium dioxide nanoparticles in the fish gelatin layer of the bilayers. Gelatin/agar bilayer film was produced from the monolayers using the casting method in two steps and their microstructural, physical, mechanical and optical characteristics were studied. Results showed that the addition of TiO2 decreased water vapor permeability of the bilayers more than 30%, upon increasing TiO2 content to 2 (g/100 g). However, swelling ratio and moisture content increased with the increase in the nano-TiO2 content, probably due to the hydrophilic nature of the TiO2 nanoparticles. The tensile strength of the bilayer films increased from 10.80 to 13.91 MPa upon increasing nano-TiO2 content from 0 to 0.5 (g/100 g); however, tensile strength decreased with further increase of the nanoparticle concentration. In addition, the metallic nature of nano-TiO2 considerably improved the barrier properties of the bilayer films against UV light at low concentration, while it increased their opacity. This property might help in the preservation of light-sensitive foods, but more studies on real food systems are required.
  • The Iranian Caviar and its Substitutes in the World Market

    Adeli, Afshin; Namdar, Mahya (2015)
    “Roe” is a general term for fish eggs (hard roe) or the milt of male fish (soft roe), but “caviar” refers to a valuable and rare food item that is obtained by processing of ova from some fishes, the most valuable of which is from sturgeons (Martin et al., 2000; Keyvan, 2004). Roe is marketed fresh, frozen, in brine, smoked and canned (Monfort, 2002). In the international market, only salted roe of about 20 species among sturgeons (Acipencer and Huso) and paddlefish (Polyodon and Psephurus) are sold as caviar (Monfort, 2002). Roes from fishes other than sturgeons are sometimes referred as “caviar substitutes”. However, the term “roe” and “caviar or kaviar” are sometimes used synonymously. For instance, salmon roe (red caviar) and lumpfish caviar (Bledsoe et al., 2003). At present, the eggs of more than 38 species other than sturgeon are used to produce substitutes (Bronzi and Rosenthal, 2014). Having the valuable fatty and amino acids needed for body general metabolism and especially nervous system (Motallebi and Ahari, 2011) as well as being effective in prevention of diseases such as arthritis, gastrointestinal disease and cancer (Behbahani, 2010). Caviar is considered as tonic food that can relieve the effect of anesthesia needed in surgical operation as well as patients with infectious diseases (Motallebi and Ahari, 2011).
  • Temporal Variation of Catch rate and Length Frequency of Clupeonella cultriventris (Nordmann, 1840) in Southwest of the Caspian Sea (Bandar-e Anzali).

    Koohani, Majid; Paighambari, Seyed Yousef; Adeli, Afshin; Ghorbani, Ali Asghar (2012)
    Temporal Variation of Catch rate and Length Frequency of Clupeonella cultriventris was studied in the fishing grounds of Guilan province during 1st January to 10th December 2011. There was a significant difference between the mean CPUE of different depths and seasons. The total lengths of 921 specimens were recorded during the study period ranged from 69 mm to 139 mm. The contribution of Common Kilka (C. cultriventris caspia), Big-eye Kilka (C. grimmi) and Anchovy Kilka (C. engrauliformis) from the total catch was 97.6, 1.08 and 1.3 %, respectively. Length–frequency distribution of the C. cultriventris had differed significantly among the examined seasons for seasonal variation in the size structure of their populations. The present study indicates that the season is important determinant of the capture rate and the size composition of lift net catches. Lowest CPUE was caught in spring season and highest was summer. The data collected in this study can be used as the basis for a long-term stock monitoring program in the region.