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dc.contributor.authorGonzales-Plasus, Maria Mojena G
dc.contributor.authorHaga, Yutaka
dc.contributor.authorKondo, Hidehiro
dc.contributor.authorHirono, Ikuo
dc.contributor.authorSatoh, Shuichi
dc.coverage.spatialJapanen_US
dc.date.accessioned2021-04-21T10:01:50Z
dc.date.available2021-04-21T10:01:50Z
dc.date.issued2019-07-01
dc.identifier.urihttp://hdl.handle.net/1834/17938
dc.description.abstractThe low production of hypotaurine from cysteine but a significantly high taurine deposition in common carp led to the hypothesis that this species utilizes an alternative pathway other than the cysteine sulfinic acid pathway. Cysteamine pathway is common in mammals but not in other animals such as birds, invertebrates, and fishes. The cloned cysteamine dioxygenase (ADO) cDNA in common carp consists of 790 nucleotide bases with 260 deduced amino acid sequence. The conserved domain is the DUF1637 which has a conserved tyrosine and cysteine residues and the presence of three predicted N-glycosylation sites. Phylogenetic analysis using neighbor joint method indicated that ADO in common carp branched after Sinocyclocheilus rhinocerous. ADO was expressed in hepatopancreas, brain, gill, intestine, and muscle of common carp. The hepatopacreas had a significantly higher gene expression level than the other organs examined. The present results suggest that ADO is present in common carp.
dc.language.isoenen_US
dc.relation.urihttp://www.palawanscientist.org/research_journals/thepalawanscientist_vol11_2019/2a%20Plasus.pdfen_US
dc.titleMolecular characterization and tissue distribution of cysteamine dioxygenase (ADO) in common carp Cyprinus carpioen_US
dc.typeJournal Contributionen_US
dc.bibliographicCitation.titleThe Palawan Scientisten_US
dc.bibliographicCitation.volume11en_US
dc.description.statusPublisheden_US
dc.format.pagerange17-28en_US
dc.subject.asfaASFA_2015::M::Molecular structureen_US
dc.subject.asfaASFA_2015::E::Enzyme activityen_US
refterms.dateFOA2021-04-21T10:01:51Z
html.description.abstractThe low production of hypotaurine from cysteine but a significantly high taurine deposition in common carp led to the hypothesis that this species utilizes an alternative pathway other than the cysteine sulfinic acid pathway. Cysteamine pathway is common in mammals but not in other animals such as birds, invertebrates, and fishes. The cloned cysteamine dioxygenase (ADO) cDNA in common carp consists of 790 nucleotide bases with 260 deduced amino acid sequence. The conserved domain is the DUF1637 which has a conserved tyrosine and cysteine residues and the presence of three predicted N-glycosylation sites. Phylogenetic analysis using neighbor joint method indicated that ADO in common carp branched after Sinocyclocheilus rhinocerous. ADO was expressed in hepatopancreas, brain, gill, intestine, and muscle of common carp. The hepatopacreas had a significantly higher gene expression level than the other organs examined. The present results suggest that ADO is present in common carp.en_US
dc.description.refereedRefereeden_US


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