Surface mucous as a source of genomic DNA from Atlantic billfishes (Istiophoridae) and swordfish (Xiphiidae)
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Author
Hoolihan, John P.Perez, Nerida F.
Faugue, Ronald M.
Bernard, Andrea M.
Horn, Rebekah L.
Snodgrass, Derke
Schultz, Duane R.
Date
2009
Metadata
Show full item recordAbstract
Procedures for sampling genomic DNA from live billfishesinvolve manual restraint and tissue excision that can be difficult to carry out and may produce stresses that affect fish survival. We examined the collection of surface mucous as a less invasive alternative method for sourcing genomic DNA by comparing it to autologous muscle tissue samplesfrom Atlantic blue marlin (Makaira nigricans), white marlin (Tetrapturus albidus), sailfish (Istiophorus platypterus), and swordfish (Xiphias gladius). Purified DNA from mucouswas comparable to muscle and was suitable for conventional polymerase chain reaction, random amplified polymorphicDNA analysis, and mitochondrial and nuclear locus sequencing. The nondestructive and less invasivecharacteristics of surface mucous collection may promote increased survival of released specimens and may be advantageous for other marine fish genetic studies, particularly those involving large live specimens destinedfor release.Journal
Fishery BulletinVolume
107Issue/Article Nr
3Page Range
339-342Resource/Dataset Location
http://fishbull.noaa.gov/1073/hoolihan.pdfCollections