Development, validation, and utilization of a competitive enzyme-linked immunosorbent assay for the detection of antibodies against Brucella species in marine mammals
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Author
Meegan, JennyField, Cara
Sidor, Inga
Romano, Tracy
Casinghino, Sandra
Smith, Cynthia R.
Kashinsky, Lizabeth
Fair, Patricia A.
Bossart, Gregory
Wells, Randall
Dunn, J. Lawrence
Date
2010-11
Metadata
Show full item recordAbstract
A competitive enzyme-linked immunosorbent assay (cELISA) was developed by using a whole-cell antigen from a marine Brucella sp. isolated from a harbor seal (Phoca vitulina). The assay was designed to screen sera from multiple marine mammal species for the presence of antibodies against marine-origin Brucella. Based on comparisons with culture-confirmed cases, specificity and sensitivity for cetacean samples tested were 73% and 100%, respectively. For pinniped samples, specificity and sensitivity values were 77% and 67%, respectively. Hawaiian monk seal (Monachus schauinslandi; n = 28) and bottlenose dolphin (Tursiops truncatus; n = 48) serum samples were tested, and the results were compared with several other assays designed to detect Brucella abortus antibodies. The comparison testing revealed the marine-origin cELISA to be more sensitive than the B. abortus tests by the detection of additional positive serum samples. The newly developed cELISA is an effective serologic method for detection of the presence of antibodies against marine-origin Brucella sp. in marine mammals.Journal
Journal of Veterinary Diagnostic InvestigationVolume
22Issue/Article Nr
6Page Range
856-862Resource/Dataset Location
http://vdi.sagepub.com/content/22/6/856.longae974a485f413a2113503eed53cd6c53
10.1177/104063871002200603
Scopus Count