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dc.contributor.authorMohammad Nejad Shamoushaki, Majid
dc.date.accessioned2021-06-24T17:38:37Z
dc.date.available2021-06-24T17:38:37Z
dc.date.issued2010
dc.identifier.urihttp://hdl.handle.net/1834/34452
dc.description.abstractThe acute toxicity and effects of diazinon on some haematological parameters ofkutum (Rutilus frisii kutum, Kamensky, 1901) weighing 613.33 g±157.06 g werestudied under static water quality conditions at 15°C ± 2ºC in winter and spring2009. The effective physical and chemical parameters of water were pH= 7-8.2,dh= 300mg/L (caco3), DO= 7 ppm and T= 15°C±2ºC. The first test was primarilyto determine the effects of acute toxicity (LC5096 h) of the agricultural toxicantdiazinon (emulsion 60%) on kutum male brood stocks. For this purpose, 4treatments were used to test toxicity; each treatment was repeated in 3 tanks with 9fish per treatment and with 180 litres water capacity. After obtaining the finalresults, the information was analysed statistically with Probit version 1.5 (USEPA,1985), and we determined the LC10, LC50 and LC90 values at 24 hours, 48 hours,72 hours and 96 hours; the maximum allowable concentration value (LC5096 hdivided by 10) (TRC, 1984); and the degree of toxicity. The second stage of testingconsists of four treatments: LC0= 0 as experimental treatment, treatment A with aconcentration of LC1= 0.107 mg/L, treatment B with concentration of LC5= 0.157mg/L, treatment C with concentration of MAC value= 0.04 mg/L. Male broodstocks of kutum were treated with these concentrations for 45 days. Experimentswere carried out under static conditions based on the standard TRC, 1984 methodover 45 days. Our results show that long-term exposure to diazinon causes adecrease in the erythrocyte count (RBC), haemoglobin (Hb), haematocrit (PCV),mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), meancorpuscular haemoglobin concentration (MCHC), leucocyte count (WBC),lymphocyte, testosterone, iron (Fe), sodium (Na), lactate dehydrogenase (LDH),and cholinesterase (CHeS). In addition, diazinon also causes an increase inprolymphocyte, aspartate aminotransferase (AST), cholesterol, alkalinephosphatase (ALP) and adrenaline (P<0.05). There are no significant effects onmonocyte, eosinophil, magnesium (Mg), chloride (Cl), glucose (BS), urea (BUN),uric acid (U.A), triglyceride (TG), calcium (Ca), albumin (Alb), total protein (TP),cortisol, noradrenaline and high density lipoprotein (HDL) levels in kutum malebrood stocks (P>0.05). Pathology results showed toxin diazinon no effect onaverage weight and fish body length, the average weight of heart, brain, spleen,liver, kidney and liver index but caueses decrease of gonad weigth and gonadindex and also, cause complications of tissue necrosis, vascular congestion,inflammation in the liver, a sharp reduction in the number of glomeruli, necrosis,vascular congestion and haemorage in the kidney, capsule thickening and fibrosis,atrophy, vascular congestion, macrophages release increased, increasing sedimentHemosiderine and thickening of artery walls in the spleen, atrophy, fibrosis andnecrosis in testis , vascular congestion, increased distance between themyocardium and fibrous string in heart and neuronal loss, vascular congestion andedema in the brain of kutum male brood stocks.
dc.formatapplication/pdf
dc.format.mimetypeapplication/pdf
dc.language.isofa
dc.publisherIslamic Azad University, Science and Research Branch, Tehran, Fisheries
dc.relation.urlhttp://www.srbiau.ac.ir/
dc.subject.otherAquaculture
dc.subject.otherBiology
dc.subject.otherFisheries
dc.titleEffect of organophosphate, diazinon on some immunophysiological, haematological and histological variable of Rutilus frisii kutum (Kamensky,1901)
dc.typethesis
dc.description.degreePhD
dc.description.notesAdvisors: Mehdi Soltani, A. Kamali. Counsellors: Issa Sharif Poor
dc.format.pages129
refterms.dateFOA2021-06-24T17:38:37Z
dc.source.legacyrecordurlhttp://aquaticcommons.org/id/eprint/19195
dc.source.legacydepositorid17408
dc.source.legacylastmod2016-02-06 09:56:30
dc.source.legacyid19195
dc.source.legacyagencyIslamic Azad University, Science and Research Branch, Tehran


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