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Antiviral activity of Sargassum fluitans seaweed against echovirus 9, coxsackievirus A16 and coxsackievirus A24
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Author
Ponce Rey, Liena de ReglaSpengler Salabarría, Iraida
Rodeiro Guerra, Idania
Roque Quintero, Annele
del Barrio Alonso, Gloria del Carmen
Resik Aguirre, Sonia
Date
2021Creative Commons
Attribution-NonCommercial-NoDerivatives 4.0 International
Metadata
Show full item recordAlternative Title
Actividad antiviral del alga Sargassum fluitans (Børgesen) Børgesen 1914, frente al echovirus 9, el coxsackievirus A16 y el coxsackievirus A24Abstract
Enteroviruses cause human diseases such as Mouth, Hand and Foot Syndrome, hemor rhagic conjunctivitis, aseptic meningitis, and viral meningoencephalitis. As yet, there is not specific therapy for most enteroviruses infections. The brown seaweeds of Sargassum genus synthesize a great variety of metabolites, which make them potential sources of compounds with antiviral activities. This study aimed to evaluate the in vitro antiviral activity of a hydro-alcoholic extract of Sargassum fluitans against three human enteroviruses: coxsackievirus A16, echovirus 9 and coxsackievirus A24. Cytotoxicity of the extract was evaluated in Vero, RD and Hep-2 cells by MTT method. Antiviral activity (EC50) was assessed by cytopathic effect inhibition in cells. Extracellular virucidal activity and reduction of viral yield were determined by endpoint viral titration assay. The antiviral activity was characterized by a time of addition assay. The extract showed antiviral inhibi- tory activity against all tested viruses. The extract also exhibited a virucidal effect against E9 and CVA16 and reduced the formation of infective particles in the cells in more than three logs. The extract was able to inhibit earlier and late stages in the enterovirus replication cycle. In conclusion, the present study demonstrated the effective in vitro antiviral activity of the brown seaweed Sargassum fluitans against clinically relevant enteroviruses, supporting their use as potential source of antiviral compounds.Los enterovirus causan enfermedades humanas tales como el Síndrome de Mano, Pie y Boca, la conjuntivitis hemorrágica y la meningoencefalitis viral. Hasta el momento no existe una terapia específica para la mayoría de las infecciones por enterovirus. Las algas pardas del género Sargassum sintetizan una gran variedad de metabolitos, los cuales les convierten en fuentes potenciales de compuestos con actividad antiviral. Este estudio tuvo como objetivo evaluar la actividad antiviral in vitro de un extracto hidroalcohólico de Sargassum fluitans contra tres enterovirus humanos: coxsackievirus A16, echovirus 9 and coxsackievirus A24.La citotoxicidad del extracto se evaluó en las células Vero, RD y Hep-2 mediante el método del MTT. La actividad antiviral (CE50) se evaluó mediante la inhibición del efecto citopático en las células. La actividad virucida extracelular y la reducción del número de viriones se determinaron mediante ensayo de titulación viral de punto final. La actividad antiviral se carac- terizó mediante un ensayo de tiempo de adición. El extracto mostró actividad antiviral inhibitoria contra todos los virus probados. El extracto también exhibió efecto virucida contra el echovirus 9 y el coxsackievirus A16 y redujo la formación de partículas virales infectivas en las células en más de tres logaritmos. El extracto fue capaz de inhibir las etapas tempranas y tardías del ciclo replicativo. Se concluye que el pre- sente estudio demostró la efectiva actividad antiviral in vitro del alga parda Sargassum fluitans contra enterovirus clínicamente relevantes, apoyando su uso como fuente potencial de compuestos antivirales.
Journal
Revista de Investigaciones MarinasVolume
41Issue/Article Nr
1Page Range
pp.43-55Collections
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- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International